Cooperation of TGF-β and Notch in the induction of intestinal metaplasia and cancer stem cells of the esophagus and stomach

人胃癌标本的免疫组织化学显示，只有低分化的胃癌细胞表达干细胞标记物CD133。在胃癌细胞系中也观察到了同样的趋势。我们建立了稳定表达多西环素诱导型Notch1 siRNA载体的CD133表达胃癌细胞系KATOIII。对该细胞和其他细胞株的分析表明，CD133是由Notch1通过RBP-JK直接调控的。我们还发现，Notch1调节这些细胞的增殖和肿瘤形成。

Immunohistochemistry of human gastric cancer specimens revealed that only poorly differentiated gastric cancer cells express the stem cell marker CD133. The same tendency was observed in gastric cancer cell lines. We established a CD133 expressing gastric cancer cell line KATOIII that stably express doxycycline inducible Notch1 siRNA vector. Analysis using this and other cell line revealed that CD133 is directly regulated by Notch1 through RBP-Jk. We also found out that Notch1 regulates proliferation and tumorigenesis in these cells.