The regulation of lympho-hematopoiesis by bone marrow niches

我们产生了允许选择性消融骨髓内CAR细胞的小鼠，并确定了CAR细胞作为造血干细胞（HSC）和淋巴造血祖细胞的小生境的体内功能。体内CXC趋化因子配体（CXCL）12丰富的网状（CAR）细胞的短期消融严重损害了SCF和CXCL 12的产生，并导致循环淋巴和红系祖细胞的显着减少。来自CAR细胞耗尽小鼠的HSC的数量和细胞大小减少，更加静止，并且早期髓样选择基因的表达增加。因此，CAR细胞是CXCL12和SCF的主要生产者，并且是HSC和淋巴样和红系祖细胞增殖以及维持HSC处于未分化状态所需的。

We generated mice that allowed selective ablation of CAR cells within bone marrow and determined the in vivo function of CAR cells as a niche for hematopoietic stem cells (HSCs) and lympho-hematopoietic progenitors. Short-term ablation of CXC chemokine ligand (CXCL)12-abundant reticular (CAR) cells in vivo severely impaired the production of SCF and CXCL12, and led to a marked reduction in cycling lymphoid and erythroid progenitors. HSCs from CAR cell-depleted mice were reduced in number and cell size, were more quiescent and had increased expression of early myeloid selector genes. Thus, CAR cells are the major producer of CXCL12 and SCF and are required for proliferation of HSCs and lymphoid and erythroid progenitors as well as maintenance of HSCs in an undifferentiated state.