Relationship between inhibition of ID1 expression and induction of irreversible suppression of cell proliferation in HepG2 hepatoma cells treated with HGF

HGF处理的HepG2肝癌细胞中ID1表达抑制与诱导细胞增殖不可逆抑制的关系

• 批准号: 22570137
• 负责人: TANAKA Toshiaki
• 金额: $ 3万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22570137/
• 关键词: ホルモンと生理活性物質; 肝細胞増殖因子; 癌; ID1; 不可逆的増殖停止; Skp2; Myc; 遺伝子発現; エピジェネティクス; シグナル伝達; 除放剤; 転写制御; プロモーターアッセイ; HepG2

HGF treatment of HepG2 hepatoma cells decreases expression of an oncogene ID1. This study showed that the decrease in ID1 is regulated through downregulation of Skp2, a component of E3 ubiquitin ligase, in the presence of HGF. However, Skp2 was involved in the regulation of ID1 expression by regulating transcription activity of Myc independently of ubiquitination. This study also showed that G9a histone methyltransferase, which expression may be regulated through Skp2/Myc/ID1, is involved in the alteration of H3K9me3 localization in cell nuclei induced by HGF, leading to the irreversible suppression of cancer cell proliferation by HGF.

HGF治疗HepG2肝癌细胞可降低致癌基因ID1的表达。本研究表明，在HGF存在下，通过下调E3泛素连接酶的组分Skp2来调节ID1的减少。然而，Skp2通过独立于泛素化而调节Myc的转录活性参与ID1表达的调控。本研究还发现，G9a组蛋白甲基转移酶可能通过Skp2/Myc/ID1调控表达，参与HGF诱导H3K9me3在细胞核中的定位改变，从而导致HGF对癌细胞增殖的不可逆抑制。

项目成果

ツメガエル初期発生過程における新規CdkインヒビターInk4Xの形態形成への係わりの解析

新型Cdk抑制剂Ink4X参与非洲爪蟾早期发育过程形态发生的分析

• 发表时间: 2010
• 作者: 仁禮綾香;戸井田英俊;喜多村直実;田中利明
• 通讯作者: 田中利明

What we can do with over 300 billion bases of RNA-seq data: The case of Xenopus laevis genome project.

我们可以利用超过 3000 亿个碱基的 RNA-seq 数据做什么：非洲爪蟾基因组计划的案例。

• 发表时间: 2013
• 作者: T.Kwon;S.Takahashi;T.Tanaka;H.Noguchi;A.Toyoda;A.Fujiyama;Y.Suzuki;N.Ueno;M.Taira;J.B.Wallingford;E.M.Marcotte.
• 通讯作者: E.M.Marcotte.

unctional analyses of a novel insulin like factor that are required for neural development.

对神经发育所需的新型胰岛素样因子进行功能分析。

• 发表时间: 2012
• 作者: Y.Haramoto;S.Takahashi;Y.Onuma;Y.Ito;M.Asashima
• 通讯作者: M.Asashima

Greatwall kinase and cyclin B-Cdk1 are both critical cocnstituents of MPF

长城激酶和细胞周期蛋白B-Cdk1都是MPF的关键组成部分

• 发表时间: 2011
• 作者: M.Hara;Y.Abe;T.Tanaka;E.Okumura;T.Kishimoto
• 通讯作者: T.Kishimoto

Greatwall kinase and cyclin B-Cdkl are both critical cocnstituents of MPF

长城激酶和细胞周期蛋白B-Cdkl都是MPF的关键组成部分

• 发表时间: 2011
• 作者: M.Hara;Y.Abe;T.Tanaka;E.Okumura;T.Kishimoto
• 通讯作者: T.Kishimoto