Basic research for development of novel switch part on conformation and delivery of nucleic acid

新型核酸构象和递送开关部件开发的基础研究

• 批准号: 22590039
• 负责人: FUJII Satoshi
• 金额: $ 3万
• 依托单位: University of Shizuoka
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590039/
• 关键词: 生物物理化学; X線結晶解析; 薬物送達; 金属配位様式

The nucleic acid molecule responds to the existing environment like the metal concentration and forms the various structures. The iron storage protein adopts the shell structure like to liposome and becomes a novel drug carrier to deliver nucleic acid and to release it at the target cell. The new crystal lattice structure of the candidate protein has been determined in six forms, in which the metal ions such as ferric, zinc and cadmium ions are doped. The highly solvated packing of the dodecamer shell is suitable for the crystallographic study of the metal ion uptake pathway.

核酸分子响应于金属浓度等存在的环境，形成各种结构。铁储存蛋白具有类似脂质体的壳层结构，是一种新型的药物载体，可将核酸递送到靶细胞并释放。候选蛋白质的新晶格结构已被确定为六种形式，其中掺杂了铁，锌和镉离子等金属离子。十二聚体壳层的高度溶剂化堆积适合于金属离子摄取途径的晶体学研究。

项目成果

1,1',1",1"'-[Porphyrin-5,10,15,20-tetrayltetrakis(3,1-phenylenemethylene)]tetraquinolinium etrabromide

1,1,1",1"-[卟啉-5,10,15,20-四基四(3,1-亚苯基亚甲基)]四溴化四喹啉

• 发表时间: 2010
• 期刊: Molbank
• 影响因子: 0.6
• 作者: Y.Ishikawa;T.Yamashita;S.Fujii;T. Uno
• 通讯作者: T. Uno

A new crystal lattice structure of Helicobactor pylori neutrophil- activating protein (HP-NAP)

幽门螺杆菌中性粒细胞激活蛋白（HP-NAP）的新晶格结构

• 发表时间: 2012
• 期刊: Acta Cryst
• 作者: O.Tsuruta;H.Yokoyama;S.Fujii
• 通讯作者: S.Fujii

Crystal Structure of a Membrane Stomatin-Specific Protease in Complex with a Substrate Peptide

• DOI: 10.1021/bi300098k
• 发表时间: 2012-05-08
• 期刊: BIOCHEMISTRY
• 影响因子: 2.9
• 作者: Yokoyama, Hideshi;Takizawa, Naoto;Fujii, Satoshi
• 通讯作者: Fujii, Satoshi

ヘリコバクターピロリ好中球活性化タンパク質による金属イオンの貯蔵・放出の構造基盤～金属注入法の違いによる配位様式の差異～

幽门螺杆菌中性粒细胞激活蛋白储存和释放金属离子的结构基础~不同金属注入方式导致配位模式的差异~

• 发表时间: 2011
• 作者: K.Ohara;R.Mitsumori;他4名;西村太一;鶴田修,横山英志,藤井敏
• 通讯作者: 鶴田修,横山英志,藤井敏

Binding mode prediction and inhibitor design of anti-influenza virus diketo acids targeting metalloenzyme RNA polymerase by molecular docking

• DOI: 10.6026/97320630006221
• 发表时间: 2011-01-01
• 期刊: BIOINFORMATION
• 影响因子: 1.9
• 作者: Ishikawa, Yoshinobu;Fujii, Satoshi
• 通讯作者: Fujii, Satoshi