Role of tight junction (claudin) in the regulation of endocochlear potential

紧密连接（紧密连接蛋白）在耳蜗内电位调节中的作用

• 批准号: 22590217
• 负责人: KUBOTA Takahiro
• 金额: $ 2.91万
• 依托单位: Osaka Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590217/
• 关键词: 内リンパ腔電位; クローディン; タイト結合; 血管条辺縁細胞; Ca2+濃度; 蝸牛内リンパ腔電位; クロディーン; Ca2+; 末梢性感音難聴; ノックアウトマウス; 急性虚血; キメラマウス; Ca^<2+>; 相同組み換え; ES細胞

It had been reported that mutatios in the gene encoding claudin-14 cause deafness and nephrolithiasis. In this experiment, we made the claudin-14 knockout mice to examine the functional role of tight junctions in the endolyphatic surface cells. The obtained experimental results were as follows; 1) endocochlear potential (EP) in the endolymph in knockout mice was lower than that in wild type mice. In the preset, we are trying to measure the H+, K+, Na+,Ca2+ concentrations in endolymph. All above experimental results will be published in the next year.

据报道，编码claudin-14的基因突变可导致耳聋和肾结石。在本实验中，我们制作了claudin-14基因敲除小鼠，以研究紧密连接在内淋巴表面细胞中的功能作用。实验结果表明：（1）基因敲除小鼠耳蜗内电位（EP）低于野生型小鼠。本实验测定了内淋巴液中H+、K+、Na+、Ca ~（2+）的浓度。以上实验结果将于明年发表。

项目成果

A cancer vaccine: Specific rejection of i.p. or i.m. transplanted tumor cells by cytotoxic T lymphocytes (CTLs) from mice that had rejected intradermally (i.d.) injected tumor cells. J Physiol Sci2011; 61 Suppl 1,S252

癌症疫苗：腹腔注射特异性排斥反应

• 发表时间: 2011
• 作者: Katafuchi T.;et al;神林康弘;Maeda S. et.al.
• 通讯作者: Maeda S. et.al.

A stable ATP binding to the nucleotide binding domain is important for reliable gating cycle in an ABC transporter CFTR.

• DOI: 10.1007/s12576-010-0102-2
• 发表时间: 2010-09
• 期刊: The journal of physiological sciences : JPS
• 作者: Shimizu H;Yu YC;Kono K;Kubota T;Yasui M;Li M;Hwang TC;Sohma Y
• 通讯作者: Sohma Y

Expression of the GABAA receptor/Chloride Channel in murine spermatogenic cells

• DOI: 10.14670/hh-26.95
• 发表时间: 2011-01-01
• 期刊: HISTOLOGY AND HISTOPATHOLOGY
• 影响因子: 2
• 作者: Kanbara, Kiyoto;Mori, Yoshiaki;Otsuki, Yoshinori
• 通讯作者: Otsuki, Yoshinori

HLA-B62 as a Possible Ligand for the Human Homologue of Mouse Macrophage MHC Receptor 2 (MMR2) on Monocytes

HLA-B62 作为单核细胞上小鼠巨噬细胞 MHC 受体 2 (MMR2) 的人类同源物的可能配体

• 发表时间: 2010
• 期刊: Gene
• 影响因子: 3.5
• 作者: Shimizu T;et al.
• 通讯作者: et al.

Membrane potential moduration of ionomycin-stimulated Ca2+ entry via Ca2+/H+ exchange and SOCin rat submandibular acinar cells.

通过Ca2/H交换和大鼠下颌下腺泡细胞中的SOC来调节离子霉素刺激的Ca2进入的膜电位。

• 发表时间: 2010
• 期刊: J Physio Sci
• 作者: Yoshida H;Hirano C;Shimamoto C;Daikoku E;Kubota T;Sugita M;Shiba Y;Nakahari T
• 通讯作者: Nakahari T