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Exploring the possibilities of genome recombination

Exploring the possibilities of genome recombination
探索基因组重组的可能性
批准号:
22655053
负责人:
ATOMI Haruyuki
金额:
$2.19万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2010
资助国家:
日本
项目状态:
已结题
起止时间:
2010 至 2011

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中文摘要
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英文摘要
Focusing on the high transformation efficiency of the hyperthermophilic archaeon Thermococcus kodakarensis, we explored the possibilities of performing wide-scale DNA recombination. Mutant strains displaying uracil or tryptophan auxotrophy were used as host cells, and selection was based on their prototrophy. We were able to obtain prototrophs using the genomic DNA of wild-type T. kodakarensis. By inserting a small DNA fragment from the DNA donor beforehand, we were able to obtain transformants using genomic DNA from other hyperthermophiles.
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For mate-driven growth coupled with hydrogen production
用于配偶驱动的生长和氢气生产
DOI: --
发表时间: 2010
期刊: Nature
影响因子: 64.8
作者: [Kim, Y. J., Lee, H. S., Kim, E. S., Bae, S. S., Lim, J. K., Matsumi, R., Lebedinsky, A. V., Sokolova, T. G., Kozhevnikova, D. A., Cha, S. S., Kim, S. J., Kwon, K. K., Imanaka, T., Atomi, H., Bonch-Osmolovskaya, E. A., Lee, J. H., Kang, S. G]
通讯作者: S. G
Genomics of thermophilic bacteria and archaea
嗜热细菌和古细菌的基因组学
DOI: --
发表时间: 2012
期刊:
影响因子: --
作者: [Sato, T, Atomi, H]
通讯作者: H
DOI: 10.1002/9780470015902.a0021900
发表时间: 2010-09
期刊:
影响因子: --
作者: [Takaaki Sato;H. Atomi]
通讯作者: Takaaki Sato;H. Atomi
超好熱菌Thermococcus kodakarensisにおけるペントース代謝経路の改変
超嗜热菌柯达卡伦西斯 (Thermococcus kodakarensis) 戊糖代谢途径的修饰
DOI: --
发表时间: 2011
期刊:
影响因子: --
作者: [松原正晃, 福田青郎, 石井浩子, 松宮芳樹, 跡見晴幸, 今中忠行]
通讯作者: 今中忠行
14
    Determining the regulons and their functions in hyperthermophiles
    • 批准号:
      21350092
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $12.23万
    • 财政年份:
      2009
    • 负责人:
      ATOMI Haruyuki
    • 依托单位:
    Novel metabolic pathway discovery based on contradictions between genome data and biochemical properties
    • 批准号:
      19310126
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $13.23万
    • 财政年份:
      2007
    • 负责人:
      ATOMI Haruyuki
    • 依托单位:
    Elucidation of gene regulation systems based on genome analysis
    • 批准号:
      17350083
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $9.66万
    • 财政年份:
      2005
    • 负责人:
      ATOMI Haruyuki
    • 依托单位:
    Development of a system for enhancing protein thermostability in vivo using hyperthermophiles
    • 批准号:
      15350100
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $9.73万
    • 财政年份:
      2003
    • 负责人:
      ATOMI Haruyuki
    • 依托单位:
    海外基金