Exome sequencing of drug-metabolizing enzyme and transporter (DMET) genes and immune related genes in cynomolgus macaque.

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The cynomolgus macaque has emerged as an important experimental animal model. Here we tried to develop the detection method for encompassing SNPs of the protein-coding region (exomes) of cynomolgus macaque by next-generation sequencer. The DNA fragments encoding exomes were extracted by SeqCapEZ, and the nucleotides sequences were determined by IonPGM. (1) DMET genes:SNP information was extracted by mapping the sequences into rhesus macaque genome sequence (RheMac2). From this procedure, 767 and 553 non-synonymous substitution were detected in 15 Vietnam and 15 Philippine animals, respectively. Of them, 187 non-synonymous SNPs were detected in both populations. (2) Immune related genes:SNP information was extracted by mapping the sequences into RheMac2 and human genome sequence. As a result of analysis, we identified four candidate SNPs which divided into penotype A and B. These findings will provide for construction of SNP database and the typing system in cynomolgus macaques.