Analysis of polyoma virus related tumor: Merlkel cell polyomavirus infection and tumorgenesis

最近在默克尔细胞癌 (MCC) 中发现了默克尔细胞多瘤病毒 (MCPyV)，默克尔细胞癌是一种发生在阳光照射皮肤中的侵袭性癌症。我们对各种皮肤肿瘤组织（包括 MCC 和其他与阳光照射相关的皮肤肿瘤）中的 MCPyV 拷贝数进行了定量分析。在常规 PCR 分析中，在所有 9 个 MCC、1 个基底细胞癌和 3 个光化性角皮炎中均检测到了 MCPyV DNA。在大多数 MCC 组织中，每个单倍体基因组的病毒拷贝数估计约为 1，并且通过数字 PCR 技术，MCC 和 AK 组之间存在显着差异。 PCR 阳性 BCC 组织显示出与 MCC 组织相似的病毒载量。使用针对 MCPyV T 抗原的单克隆抗体进行的免疫组织化学显示，在大多数高病毒载量肿瘤组中，核定位呈阳性，但在低病毒载量或 PCR 阴性肿瘤组中则不然。这些结果表明，MCPyV 感染可能与少数日晒皮肤肿瘤有关。

Merkel cell polyomavirus (MCPyV) has recently been identified in Merkel cell carcinoma (MCC), an aggressive cancer that occurs in sun-exposed skin. We performed quantitative analyses of the MCPyV copy number in various skin tumor tissues, including MCC and other sun exposure-related skin tumors. In a conventional PCR analysis, MCPyV DNA was detected in all 9 MCCs, 1 Basal cell carcinoma, and 3 Actinic Keraotis. The viral copy number per haploid genome was estimated to be around 1 in most MCC tissues, and there were marked differences between the MCC and AK groups by digital PCR technology. PCR-positive BCC tissue showed a similar viral load as MCC tissue. Immunohistochemistry with a monoclonal antibody against the MCPyV T antigen demonstrated positive nuclear localization in most of the high-viral-load tumor groups, but not in the low-viral-load or PCR-negative tumor groups. These results demonstrated that MCPyV infection is possibly involved in a minority of sun-exposed skin tumors.