Non-biased expression analysis to search for molecules associated with amyloid-beta induced hyperphosphorylation of tau

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Senile plaque and neurofibrillary tangle are pathological hallmarks of brains of Alzheimer's disease. Senile plaque consists of amyloid-beta (Abeta) and neurofibrillary tangle is composed of hyperphosphorylated tau. The mechanism by which Abeta induces hyperphosphorylation of tau remains unclear. In this study, we addressed this question by establishing coculture system in which Abeta-induced hyperphosphorylation of tau is recapitulated. Total RNA was extracted from primary cultured neurons in which hyperphosphorylation of tau is induced by physiologically secreted Abeta by coculture system. By conducting unbiased examination using RNA expression array, we found that expressions in several genes are altred in condition where hyperphosphorylation of tau occurred in primary neurons. Bioinformatics analysis revealed that genes of altered expression affect insulin signaling.