Application of a cell-free protein display technology to the high-throughput functional proteomic screen for regulators of neuron-glia interaction

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Fluorophore-assisted light inactivation (FALI) in combination with a cell-based assay is a uniquemethod for functional proteomic screen to search for regulators of a specific cellular function. One of the major problems with the method is the construction of antibody libraries that contain high-affinity binders to cell surface membrane proteins as potential targets. To overcome barriers to efficiency, we used a cell-free antibody display method. We tried to optimize the in vitro expression of the recombinant antibody that is linked to its genotype and the neuregulin-erbB model system for pilot-scale screening ofpotential regulators for neuron-glia interaction