Establishment of human liver chimeric mice with normal immune system for hepatitis viral infection

病毒性肝炎是一种非常严重的肝脏疾病。由于肝炎病毒不能感染正常的实验动物，如小鼠和大鼠，因此研究这种疾病的治疗方法很困难。因此，我们计划建立一种新的小鼠模型来研究病毒性肝炎。(1)建立了一种纯化和扩增人多能细胞肝祖细胞的新培养方法。这些细胞是人肝嵌合体小鼠供体细胞的候选细胞。(2)我们尝试将原代肝祖细胞移植到新生小鼠体内。在受体的肝和肺中检测到少量供体细胞。

Viral hepatitis is a very severe liver disease. Studies for therapies of this disease are difficult because Hepatitis viruses cannot infect to normal experimental animals such as mouse and rat. Thus, we have a plan to establish a new mouse model for the studies of viral hepatitis.(1) We established a new culture method for the purification and expansion of hepatic progenitor cells derived from human pluripotent cells. These cells are candidate of donor cells into human liver chimera mice.(2) We tried to transplant primary hepatic progenitor cells derived into neonatal mice. Small numbers of donor cells were detected in the recipient liver and lung.