Development of 3D spine analysis method and analysis of spines in prefrontal cortex

我们开发了一个数学分析程序来处理哺乳动物大脑中大量的树突棘，为许多研究人员提供了更好的脊椎分析方法。为了进一步实现三维分析，我们检查了许多荧光试剂，并发现了一种候选物质，具有强大的抗褪色特性，适合于共聚焦成像。我们还建立了大脑中催产素的精确定量方法，并制作了催产素对前额皮质棘的多电极测量系统。

We have developed a mathematical analysis program to handle vast numbers of dendritic spines in the mammalian brain, providing a better way of spine analysis for many researchers. To further enable three-dimensional analysis, we examined a number of fluorescent reagents and found a candidate substance with robust anti-fading properties suitable for confocal imaging. We also established a method for precise quantification of oxytocin in the brain and made a multi-electrode measuring system for oxytocin effects on the spines in the prefrontal cortex.