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エリスロキロチン遺伝子の胚発生過程における発現の意義の解明

阐明胚胎发育过程中红细胞素基因表达的意义

基本信息

批准号：
09877006
负责人：
安田佳子
金额：
$ 1.28万
依托单位：
Kinki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Exploratory Research
财政年份：
1997
资助国家：
日本
起止时间：
1997 至无数据
项目状态：
已结题

项目摘要

1)目的:着床後のマウス胚は卵黄嚢血管や嚢帯血管が形成されるまでは主に子宮内膜由来の脱落膜内で発生が進行する。この時期は胚にとって極度な低酸素環境であるにもかかわらず、マウス胚は急速に成長する。この脱落膜にエリスロポエチン受容体(EPR)のmRNAが発現している。一方着床後のマウス胚でもEPRmRNAはエリスロポエチン(EP)mRNAより先んじて発現し、次で両mRNAが発現する(Yasuda et al.,1993)。このことは胚発生にEPR-EPのシグナル伝達が何らかの働きを担っていることを示唆する。そこでEP-EPRシグナル伝達を断ち胚発生に及ぼす影響をみた。2)材料と方法:ICR系マウスを用いて、胚着床直後の妊娠4日母獣(膣栓発券日を妊娠0日とする)を麻酔下で開腹し、子宮を取り出し、片側の子宮内に可溶型EPR(SEPR)を、反対側に対照として生食水をあるいは変性可溶型EPR(deNSER)を注入し、子宮を腹腔に戻して閉腹した。3日後に母獣より、脱落膜体(胎児と脱落膜)を摘出し、検鏡後、実験に供した。3)結果:a)胚成長傷害:SEPRを4.0、0.4、0.08および0.04mg/mlの濃度で処理した子宮から得た脱落膜は、それぞれ88.2、79.7、57.9、37.5%の頻度で蒼白な脱落膜体が出現した。deNSERやsalineの対照の脱落膜では12.3〜0%の頻度で認められたが、SEPR処理脱落膜体と対照群との間に有意の差(p<0.001、とp<0.01)を示して高頻度に出現した。一方、胚の方は4.0、0.4、および0.08mg/mlのSEPR処理群で、対照群との間に有意差(p<0.001)を示して、胚の成長阻止が認められた。b)脱落膜の変化:脱落膜のEPシグナル遮断によって脱落膜細胞の状態を免疫組織学的に検索したところ、ライフェルト膜外にある巨細胞および2次巨細胞が傷害を受け壊死像を示した。この死をTdT検索したところ陽性反応を示した。従ってEPR-シグナル遮断によって脱落膜巨細胞がアポト-ジス死をすることが判明した。4)考察:EP-EPRシグナル伝達はマウス胚発生過程で脱落膜細胞の生存を担っている。母獣よりの血液が充分でないとEPR-EPシグナル伝達が傷害され、胚成長が2次的に阻害される。

1) objective: after implantation, the embryo, yolk, blood vessels, blood vessels, the formation of blood vessels, the origin of the intima, the origin of the exfoliated intima, the formation of blood vessels, the formation of blood vessels and the formation of blood vessels. During the period of time, the temperature of the embryo is low in the environment, and the embryo grows rapidly in the environment. The exfoliated membrane, the capacitor (EPR), the mRNA, the membrane, the capacitor (EPR), the capacitor, the recipient, the recipient, the After one side is implanted, the embryo is not seen. The embryo is EPRmRNA. The embryo is not seen. (EP) the mRNA is first seen, and the second is mRNA. It is seen (Yasuda et al.,1993). Please tell me how to give birth to the EPR-EP baby. Please EP-EPR the baby and make sure that you don't know how to make a living. 2) Materials and methods: ICR was administered to the pregnant women on the 4th day of pregnancy after implantation, the abdominal operation was performed on the 4th day of pregnancy (on the 0th day of pregnancy), the child was taken out, the tablet was filled with soluble EPR (SEPR), the anti-drug was injected into the raw water, the soluble EPR (deNSER) was injected into the abdominal cavity, and the abdominal cavity was injected into the abdominal cavity. After 3 days, the mother and the exfoliated membrane (the exfoliated membrane of the fetus) were removed and supplied to the mother. 3) results: a) the damage of embryo growth: the exfoliated membrane was obtained in SEPRL 4.0,0.4,0.08 0.04mg/ml temperature, and the exfoliated membrane was found in 77.7,57.9,37.5% temperature. DeNSER "saline" irradiation "exfoliated film", "12.3" 0% "temperature", "SEPR"exfoliated film", "light group"intentional" difference (paired LTX 0. 001, "PUBLTACHY" 0. 01) show that the high temperature shows that there is a difference. One side, embryo growth rate 4.0,0.4,4, 0.08mg/ml embryo growth prevents the recognition of pregnancy by preventing the growth of embryos. B) exfoliated membrane exfoliation: exfoliated membrane, EP membrane, exfoliated membrane Please tell me that the TdT is so bad that it will show you the opposite sex. The exfoliated membrane giant cell was blocked by EPR-, and the cell death was detected. 4) investigation: EP-EPR cells are responsible for the survival of exfoliated membrane cells during the process of embryo birth. The mother has enough blood to stop the damage caused by EPR-EP and to prevent the embryo from growing twice.