Production of Pedigreed SPF Rhesus Macaques

纯种 SPF 恒河猴的生产

• 批准号: 10332754
• 负责人: Jeffrey A Roberts
• 金额: $ 115.36万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2024-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10332754
• 关键词: AIDS/HIV problem; Alleles; Animals; Biological Assay; Breeding; Censuses; Cercopithecine Herpesvirus 1; Collection; Cytomegalovirus; DNA; Data; Databases; Dependovirus; Development; Diagnostic tests; Genes; Genetic; Genetic Markers; Genetic Structures; Genetic Variation; Genotype; Goals; HIV; HIV Infections; Haplotypes; Immune response; Immune system; Inbreeding; Infection; Inflammation; Knowledge; Laboratories; Lymphocryptovirus; Lymphocyte; Lymphoid Cell; Macaca; Macaca mulatta; Maintenance; Microsatellite Repeats; Modeling; Peripheral; Population; Primates; Production; Publishing; Recording of previous events; Research; Research Personnel; Research Project Grants; Rhadinovirus; Rhesus; Sampling; Serotyping; Simian Foamy Virus; Simian Retroviruses; Standardization; Technology; Testing; Tuberculosis; Validation; Viral; Virus; Work; animal colony; base; biobank; detection test; diagnostic assay; fecal microbiome; genetic pedigree; genetic testing; gut microbiome; host microbiome; improved; microbiome; novel; novel diagnostics; offspring; pathogen; population genetic structure; prevent; programs; recruit; screening; simian T cell lymphotrophic virus; sound; testing services; virology

Overall Abstract The CNPRC has maintained a pedigreed colony of Indian origin rhesus macaques for over 19 years. The SPF Pedigreed Indian origin rhesus colony includes both an SPF level 1 colony negative for Simian Retrovirus (SRV), Simian Immmunodeficiency Virus (SIV) and Simian T Cell Lymphotrophic Virus (STLV) Herpes B virus (B virus) and an SPF level 2 colony free of three additional viruses including include rhesus cytomegalovirus (RhCMV), simian foamy virus (SFV), and rhesus rhadinovirus (RRV). All colony animals have MHC typing for Mamu-A*01, Mamu-A*08, and Mamu-B*01 and the colony is managed to optimize genetic diversity. No additional animal recruitment necessary. This application will expand the percentage of SPF2 rhesus while maintaining the current overall colony size. The application proposes to evaluate the possible addition of Adeno-associated virus (AAV) and Lymphocryptovirus (LCV) to a subset of SPF2 animals and the addition of Mamu B*17 to the MHC typing panel. This application will continue to collect fecal microbiome and peripheral lymphoid cells as a biorepository for use by HIV/AIDS investigators. The four principal goals of this application are: Screen the current SPF2 colony to explore the potential for the addition of AAV and LCV to the excluded agents, or more simply for adding information about these infections to our database. Develop improved and expanded screening and confirmatory tests for detection of SPF agents to facilitate maintenance of the SPF colony. This work includes new assays for agents such as tuberculosis, which represents the first SPF agent. Evaluate addition of Mamu-B*17 to the currently tracked MHC alleles, Mamu-A*01, Mamu-A*08, and Mamu- B*01. Transition the colony pedigree analysis from the current microsatellite platform to the SNP panel and validate the proposed SNP panel with existing microsatellite data. Continue the collection and storage of microbiome and lymphocyte samples in the unique biorepository of SPF2 to facilitate future research projects investigating the interaction of HIV with the host microbiome and immune system.

总体摘要 CNPRC已维持一个印度恒河猴血统的殖民地超过19年。的SPF Pedigreed印度起源恒河猴菌落包括猴逆转录病毒阴性的SPF 1级菌落 (SRV)猴免疫缺陷病毒（SIV）和猴T细胞嗜酸性病毒（STLV）疱疹B 病毒（B病毒）和不含三种其他病毒（包括恒河猴巨细胞病毒）的SPF 2级菌落 （RhCMV）、猿猴泡沫病毒（SFV）和恒河猴Rhadinovirus（RRV）。所有群体动物均具有MHC分型， Mamu-A*01、Mamu-A*08和Mamu-B*01，并殖民地进行管理以优化遗传多样性。没有额外 动物招募是必要的。该应用程序将扩大SPF 2恒河猴的百分比，同时保持 目前的整体殖民地规模。该申请建议评价可能添加的腺相关病毒 (AAV)和淋巴隐病毒（LCV）的免疫应答，并向MHC中添加Mamu B*17 打字面板。该应用将继续收集粪便微生物组和外周淋巴细胞， 供艾滋病毒/艾滋病研究人员使用的生物储存库。该应用程序的四个主要目标是： 筛选当前SPF 2菌落，以探索向排除的试剂中添加AAV和LCV的可能性， 或者更简单地说，将这些感染的信息添加到我们的数据库中。 开发改进和扩大的SPF因子检测筛查和确认测试， SPF菌落的维持。这项工作包括对结核病等病原体的新测定， 表示第一SPF剂。 评价将Mamu-B*17添加到当前跟踪的MHC等位基因Mamu-A*01、Mamu-A*08和Mamu-A * 08中的情况。 B*01。将殖民地谱系分析从当前微卫星平台过渡到SNP组， 用现有的微卫星数据验证所提出的SNP小组。 继续在SPF 2的独特生物储存库中收集和储存微生物组和淋巴细胞样本 促进未来的研究项目，调查艾滋病毒与宿主微生物组和免疫系统的相互作用， 系统