Production of Pedigreed SPF Rhesus Macaques

总体摘要 中国一直是印度原产恒河猴的纯种聚居地，至今已超过19年。防晒系数 纯种印度源恒河猴群体包括对猿猴逆转录病毒阴性的SPF 1级群体 (SRV)、猿猴免疫缺陷病毒(SIV)和猿猴T细胞淋巴营养病毒(STLV)疱疹B 病毒(B病毒)和SPF 2级菌落，不含包括恒河猴巨细胞病毒在内的三种额外病毒 (RhCMV)、猴泡沫病毒(SFV)和恒河猴轮状病毒(RRV)。所有群体动物都有MHC分型 MAMU-A*01、MAMU-A*08和MAMU-B*01，并对菌落进行管理以优化遗传多样性。没有额外的费用 动物招募是必要的。此应用程序将扩大SPF2恒河猴的百分比，同时保持 当前的总菌落大小。该应用程序建议评估腺相关病毒的可能添加 对SPF2动物亚群的AAV和LCV以及在MHC中添加Mamu B*17的研究 打字面板。这项应用将继续收集粪便微生物群和外周淋巴细胞作为 供艾滋病毒/艾滋病调查人员使用的生物仓库。此应用程序的四个主要目标是： 筛选当前的SPF2菌落以探索将AAV和LCV添加到排除的制剂中的可能性， 或者更简单地说，将这些感染的信息添加到我们的数据库中。 开发改进和扩展的筛查和确认测试以检测SPF试剂，以促进 SPF菌落的维护。这项工作包括对结核病等病原体的新检测， 表示第一个SPF代理。 评估将MAMU-B*17添加到当前跟踪的MHC等位基因MAMU-A*01、MAMU-A*08和MAMU- B*01。将群体系谱分析从当前的微卫星平台过渡到SNP小组和 用现有的微卫星数据验证拟议的SNP小组。 继续收集和储存SPF2独特的生物储存库中的微生物组和淋巴细胞样本 促进将来研究艾滋病毒与宿主微生物群和免疫相互作用的研究项目 系统。

Overall Abstract The CNPRC has maintained a pedigreed colony of Indian origin rhesus macaques for over 19 years. The SPF Pedigreed Indian origin rhesus colony includes both an SPF level 1 colony negative for Simian Retrovirus (SRV), Simian Immmunodeficiency Virus (SIV) and Simian T Cell Lymphotrophic Virus (STLV) Herpes B virus (B virus) and an SPF level 2 colony free of three additional viruses including include rhesus cytomegalovirus (RhCMV), simian foamy virus (SFV), and rhesus rhadinovirus (RRV). All colony animals have MHC typing for Mamu-A*01, Mamu-A*08, and Mamu-B*01 and the colony is managed to optimize genetic diversity. No additional animal recruitment necessary. This application will expand the percentage of SPF2 rhesus while maintaining the current overall colony size. The application proposes to evaluate the possible addition of Adeno-associated virus (AAV) and Lymphocryptovirus (LCV) to a subset of SPF2 animals and the addition of Mamu B*17 to the MHC typing panel. This application will continue to collect fecal microbiome and peripheral lymphoid cells as a biorepository for use by HIV/AIDS investigators. The four principal goals of this application are: Screen the current SPF2 colony to explore the potential for the addition of AAV and LCV to the excluded agents, or more simply for adding information about these infections to our database. Develop improved and expanded screening and confirmatory tests for detection of SPF agents to facilitate maintenance of the SPF colony. This work includes new assays for agents such as tuberculosis, which represents the first SPF agent. Evaluate addition of Mamu-B*17 to the currently tracked MHC alleles, Mamu-A*01, Mamu-A*08, and Mamu- B*01. Transition the colony pedigree analysis from the current microsatellite platform to the SNP panel and validate the proposed SNP panel with existing microsatellite data. Continue the collection and storage of microbiome and lymphocyte samples in the unique biorepository of SPF2 to facilitate future research projects investigating the interaction of HIV with the host microbiome and immune system.