Production of Pedigreed SPF Rhesus Macaques

纯种 SPF 恒河猴的生产

• 批准号: 10203147
• 负责人: Jeffrey A Roberts
• 金额: $ 55.35万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2024-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10203147
• 关键词: Acquired Immunodeficiency Syndrome; Adopted; Age; Alleles; Animals; Area; Biological; Biological Assay; Breeding; Cells; Computer Models; Computerized Medical Record; Data; Data Analyses; Data Set; Databases; Detection; Freezing; Funding; Gender; Genetic; Genetic Fingerprintings; Genomics; Goals; HIV; Haplotypes; Immune; Immune system; Individual; Knowledge; Laboratories; Lymphocyte; Lymphoid; Lymphoid Cell; Macaca mulatta; Major Histocompatibility Complex; Methods; Microsatellite Repeats; Modeling; Pathogen detection; Phenotype; Population; Production; Recording of previous events; Research; Research Design; Research Personnel; Research Project Grants; Resources; Rhesus; SIV; Sampling; Single Nucleotide Polymorphism; Testing; Therapeutic Intervention; Viral; age group; aged; animal care; animal resource; base; biobank; computer program; flexibility; genetic analysis; genetic pedigree; genetic testing; gut microbiome; host microbiome; improved; microbiome; programs; recruit; response; screening; testing services; user-friendly; vaccine response; working group

Overall The CNPRC has maintained a pedigreed colony of Indian origin rhesus macaques for over 15 years. This Indian origin rhesus population has an extensive pedigree and is self-sustaining with no additional animal recruitment necessary. This application proposes to expand both the number and quality of Indian origin SPF rhesus provided to AIDS funded investigators. This expansion and improvement in animal numbers and quality will be achieved in the following way. 1. Expand production of SPF Level 2 animals by using smaller corn-cribs for smaller, more flexible breeding groups. This will provide greater control over animal production to meet investigator needs. 2. Transfer the current colony database on the CNPRC Vitals computer program to a new LabKey platform that includes electronic medical records as well as a user friendly search function. 3. Develop improved and expanded screening and confirmatory tests for detection of SPF agents. 4. Transition the colony pedigree analysis from the current microsatellite platform to the SNP panel being developed by the Genetics and Genomics Working Group (GGWC) Consortium of the CNPRC. 5. Expand our characterization of the microbiome and immune phenotype of both levels of SPF animals and provide a unique biorepository of fecal and lymphocyte samples which will facilitate future research projects investigating the interaction of HIV with the host microbiome and immune system. The inclusion of the Biorepository Core is in response to research that suggests that knowledge of an individual animal's microbiome may be as important as understanding its MHC haplotype in studying both vaccine response and therapeutic intervention in AIDS related studies.

整体 CNPRC已经维持了一个印度恒河猴的纯种群体超过15年 年这印度起源恒河猴人口有一个广泛的血统，是自我维持 不需要额外的动物招募。此应用程序建议扩展 提供给艾滋病资助研究人员的印度SPF恒河猴的数量和质量。这 扩大和提高动物数量和质量将在以下方面实现 路上了 1.扩大SPF 2级动物的生产，使用更小的玉米床， 灵活的繁殖群体。这将为动物生产提供更大的控制， 调查员需要。 2.将CNPRC Vitals计算机程序上的当前菌落数据库转移到新的 LabKey平台，包括电子医疗记录以及用户友好的搜索 功能 3.开发改进和扩大的SPF检测筛查和确认试验 剂. 4.将殖民地谱系分析从当前的微卫星平台过渡到SNP 遗传学和基因组学工作组（GGWC） CNPRC的联合体。 5.扩展我们对两种水平的微生物组和免疫表型的表征， SPF动物，并提供粪便和淋巴细胞样品的独特生物储存库， 将促进未来的研究项目调查艾滋病毒与宿主的相互作用 微生物组和免疫系统。 包括生物储藏核心是为了回应研究表明，知识 了解个体动物的微生物组可能与了解其MHC单倍型一样重要 在艾滋病相关研究中研究疫苗反应和治疗干预。