Production of Pedigreed SPF Rhesus Macaques

纯种 SPF 恒河猴的生产

• 批准号: 9416184
• 负责人: Jeffrey A Roberts
• 金额: $ 110.92万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2020-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9416184
• 关键词: Acquired Immunodeficiency Syndrome; Adopted; Age; Alleles; Animals; Area; Biological; Biological Assay; Breeding; Cells; Computer Simulation; Computerized Medical Record; Data; Data Analyses; Data Set; Databases; Detection; Freezing; Funding; Gender; Genetic; Genetic Fingerprintings; Genetic screening method; Genomics; Goals; HIV; Haplotypes; Immune; Immune system; Individual; Knowledge; Laboratories; Lymphocyte; Lymphoid; Lymphoid Cell; Macaca mulatta; Major Histocompatibility Complex; Methods; Microsatellite Repeats; Modeling; Pathogen detection; Phenotype; Population; Production; Recording of previous events; Research; Research Design; Research Personnel; Research Project Grants; Resources; SIV; Sampling; Single Nucleotide Polymorphism; Testing; Therapeutic Intervention; Viral; age group; aged; animal care; animal resource; base; biobank; computer program; flexibility; genetic analysis; genetic pedigree; gut microbiome; host microbiome; improved; microbiome; programs; public health relevance; recruit; response; screening; testing services; user-friendly; vaccine response; working group

 DESCRIPTION (provided by applicant): The CNPRC has maintained a pedigreed colony of Indian origin rhesus macaques for over 15 years. This Indian origin rhesus population has an extensive pedigree and is self-sustaining with no additional animal recruitment necessary. This application proposes to expand both the number and quality of Indian origin SPF rhesus provided to AIDS funded investigators. This expansion and improvement in animal numbers and quality will be achieved in the following way. 1. Expand production of SPF Level 2 animals by using smaller corn-cribs for smaller, more flexible breeding groups. This will provide greater control over animal production to meet investigator needs. 2. Transfer the current colony database on the CNPRC Vitals computer program to a new LabKey platform that includes electronic medical records as well as a user friendly search function. 3. Develop improved and expanded screening and confirmatory tests for detection of SPF agents. 4. Transition the colony pedigree analysis from the current microsatellite platform to the SNP panel being developed by the Genetics and Genomics Working Group (GGWC) Consortium of the CNPRC. 5. Expand our characterization of the microbiome and immune phenotype of both levels of SPF animals and provide a unique biorepository of fecal and lymphocyte samples which will facilitate future research projects investigating the interaction of HIV with the host microbiome and immune system. The inclusion of the Biorepository Core is in response to research that suggests that knowledge of an individual animal's microbiome may be as important as understanding its MHC haplotype in studying both vaccine response and therapeutic intervention in AIDS related studies.

 描述（由申请人提供）：CNPRC已经维持了一个印度恒河猴的纯种群体超过15年。该印度恒河猴种群具有广泛的谱系，并且自我维持，无需额外的动物招募。这项申请建议扩大提供给艾滋病资助研究人员的印度原产SPF恒河猴的数量和质量。动物数量和质量的扩大和提高将通过以下方式实现。1.扩大SPF 2级动物的生产，使用更小的玉米笼，更灵活的育种群体。这将提供对动物生产的更大控制，以满足研究者的需求。2.将CNPRC Vitals计算机程序上的当前菌落数据库转移到新的LabKey平台，该平台包括电子病历以及用户友好的搜索功能。3.开发用于检测SPF因子的改进和扩展的筛查和确认测试。4.将殖民地谱系分析从当前的微卫星平台过渡到CNPRC的遗传学和基因组学工作组（GGWC）联盟正在开发的SNP面板。5.扩大我们对两种SPF动物的微生物组和免疫表型的表征，并提供粪便和淋巴细胞样本的独特生物储存库，这将有助于未来研究HIV与宿主微生物组和免疫系统相互作用的研究项目。生物储存库核心的纳入是对研究的回应，该研究表明，在研究艾滋病相关研究中的疫苗反应和治疗干预时，了解个体动物的微生物组可能与了解其MHC单倍型一样重要。