Husbandry and Management Core

畜牧与管理核心

• 批准号: 10553267
• 负责人: Jeffrey A Roberts
• 金额: $ 81.97万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2024-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10553267
• 关键词: AIDS Vaccines; AIDS therapy; Acquired Immunodeficiency Syndrome; Animal Housing; Animals; Area; Biological; Breeding; Censuses; Collection; Data Set; Databases; Dependovirus; Electronics; Exclusion; Extramural Activities; Feedback; Female; Funding; Future; Genetic; Goals; Grant; HIV Infections; HIV/AIDS; Housing; Immune response; Immune system; Infant; Inflammation; Intervention; Lymphocryptovirus; Lymphocyte; Lymphoid; Lymphoid Cell; Macaca; Macaca mulatta; Modeling; Monkeys; Outcome Study; Peripheral; Physical Examination; Population; Primates; Process; Production; Records; Research; Research Personnel; Research Project Grants; Research Subjects; Rhesus; Role; Roundup; SIV; Sales; Sampling; Serotyping; Services; Specimen; System; Testing; Time; United States National Institutes of Health; Vaccines; Viral; Virus; Virus Diseases; Work; biobank; cost; cost efficient; electronic health record system; fecal microbiome; flexibility; genetic pedigree; gut microbiome; health record; host microbiome; insight; male; microbiome; pathogen; research faculty; response; social; social group; synergism

Abstract The CNPRC has maintained a breeding colony of SPF Indian-origin rhesus macaques SPF for over nineteen years. This colony has two different levels of SPF rhesus. The population of Level 1 animals are negative for 4 persistent viruses, (SIV, SRV, STLV, and B virus). Level 2 animals are negative for 7 persistent viruses (SIV, SRV, STLV, SFV, RRV, RhCMV, and B virus). The goal of the Husbandry and Management Core is to expand the number of SPF Level 2 animals to meet the needs of AIDS funds investigators investigating interaction between the host microbiome and AIDS vaccine and therapies. The percentage of SPF2 animals will be increased while maintaining the overall colony size. The Husbandry and Management Core will work with the Viral Testing Core to evaluate the possible addition of 2 more viruses (Adeno-associated virus, Lymphocryptovirus) for SPF exclusion. We will also evaluate with the Genetics Management Core the addition of Mamu B*17 to the MHC typing panel for the colony. Finally, the Husbandry and Management Core will systematically collect fecal microbiome and peripheral lymphoid cells from SPF animals at their biannual physical exams to be maintained in the biorepository for use by investigators conducting HIV/AIDS research.

摘要 CNPRC维持了一个SPF印度恒河猴SPF繁殖群， 超过十九年。这个群体有两种不同水平的SPF恒河猴。人口水平 1只动物的4种持久性病毒（SIV、SRV、STLV和B病毒）为阴性。2级动物 7种持久性病毒（SIV、SRV、STLV、SFV、RRV、RhCMV和B病毒）均为阴性。目标 畜牧和管理核心的一个重要目标是扩大SPF 2级动物的数量， 艾滋病基金调查人员的需求，调查宿主微生物组与 艾滋病疫苗和治疗。SPF 2动物的百分比将增加，同时维持 整个殖民地的大小。畜牧和管理核心将与病毒检测部门合作 核心评价可能增加的2种病毒（腺相关病毒， 淋巴隐病毒）进行SPF排除。我们还将评估与遗传管理核心 将Mamu B*17添加到殖民地的MHC分型板中。最后，畜牧业和 管理核心将系统性地收集粪便微生物组和外周淋巴细胞， 将在生物库中保存的SPF动物在其一年两次的体格检查时， 进行艾滋病毒/艾滋病研究的调查员。