AMPEROMETRIC GLUCOSE SENSOR WITH REDOX POLYMER MEDIATORS

本提案描述了新的基于酶的设计和测试 用于葡萄糖的安培式传感器之间的电子通信 实现了葡萄糖氧化酶的黄素氧化还原中心和电极 通过化学结合的供体-受体继电器网络 柔性聚合物。传感器应该有广泛的技术 在临床实验室、兽医保健等领域的应用 CARE、研究实验室和食品工业。因为 电极，可重复使用的体外血糖监测仪的研制 (用于糖尿病患者的自我监测)在近期看来是可行的 未来。聚合物继电器系统也适用于针型 可用于皮下血糖监测的探头。这个 第二阶段的拟议研究计划高度关注 通过系统研究实现稳定、灵敏、选择性高的葡萄糖传感器 新的聚合物电子接力系统，新的酶固定化方案， 以及新的电极配置和材料。

This proposal describes the design and testing of new enzyme-based amperometric sensors for glucose where the electrical communication between the flavin redox centers of glucose oxidase and an electrode is achieved via a network of donor-acceptor relays chemically bound to a highly flexible polymer. The sensors should have widespread technological applications in areas such as the clinical laboratory, veterinary health care, the research laboratory, and the food industry. Because the electrode, the development of a reusable in vitro blood glucose monitor (for self-monitoring by diabetic patients) appears feasible in the near future. The polymeric relay systems are also amenable to needle-type probes which could be useful for subcutaneous glucose monitoring. The proposed research plan for Phase II concerns the development of highly stable, sensitive and selective glucose sensors through a systematic study of new polymeric electron relay systems, new enzyme immobilization schemes, and new electrode configurations and materials.