ENZYME SENSORS - DIAGNOSIS OF ALCOHOL-RELATED PATHOLOGY

第一阶段研究阶段的目标是构建和测试新的 用于几种血清酶测定的安培生物传感器， 丙氨酸氨基转移酶、天冬氨酸氨基转移酶和Nu- 谷氨酰基转移酶，经常与酒精相关 器官损伤和酗酒。传感器是基于选择性的 检测由这些催化剂催化的反应生成的产物 血清酶。一种新的传感器设计允许其在低电压下运行 电势，其中杂散电流测量由氧化共同 避免了干扰物(例如，抗坏血酸、尿酸盐)。在第二阶段，这 技术将扩展到对其他实验室的选择性检测 酒精中毒的标志，如肌酸、尿素和谷氨酸 脱氢酶。优化后的传感器可以发挥重要作用 饮酒相关辨证的日常临床实践 器官损伤和酒精中毒治疗计划的后续研究中。

The goals of the Phase I research period are to construct and test new amperometric biosensors for the determination of several serum enzymes, alanine aminotransferase, aspartate aminotransferase, and nu- glutamyltransferase, which are frequently associated with alcohol-related organ damage and alcoholism. The sensors are based on the selective detection of products formed by reactions which are catalyzed by these serum enzymes. A novel sensor design permits its operation at low applied potentials, where spurious current measurements due to oxidation of common interferents (e.g., ascorbate, urate) are avoided. During Phase II, this technology will be extended to the selective detection of other laboratory markers of alcoholism, such as creatinine, urea, and glutamate dehydrogenase. The optimized sensors could serve an important role in the daily clinical practice of differential diagnostics of alcohol-related organ damage and in follow-up studies of alcoholism treatment programs.