AMPEROMETRIC GLUCOSE SENSOR WITH REDOX POLYMER MEDIATORS

一种新型的电流型葡萄糖传感器， 葡萄糖氧化酶的黄素氧化还原中心和 通过化学上的供体-受体中继网络实现电极 与柔性硅氧烷聚合物结合，最近在我们的 laboratories. 这种生物传感器可能被证明是适合植入 由于电子转移介体共价连接到 不溶性聚合物，不能从电极扩散出去。 拟议 第一阶段的研究计划涉及这类传感器的开发 通过以下途径：（1）合成一族聚合物 基于各种二茂铁和醌衍生物的继电器系统， 在氧化电位和再氧化葡萄糖氧化酶的能力上不同，和 硅氧烷共聚物;（2）碳糊电极的构建 含有葡萄糖氧化酶和这些新的聚合物中继系统中的每一种; (3)使用循环伏安法对这些生物传感器进行电化学测试 和恒电位程序。 重点将放在确定 基于以下的最佳电子转移中继系统 注意事项：对葡萄糖敏感，工作电位低， 对氧气不敏感，长期稳定。

A new class of amperometric glucose sensor, in which electrical communication between the flavin redox centers of glucose oxidase and an electrode is achieved via a network of donor-acceptor relays chemically bound to a flexible siloxane polymer, has recently been invented in our laboratories. This biosensor may prove to be suitable for implantation since the electron transfer mediators are covalently attached to an insoluble polymer and cannot diffuse away from the electrode. The proposed research plan for Phase I concerns the development of this class of sensor through the following approach: (1) synthesis of a family of polymeric relay systems based on various ferrocene and quinone derivatives, which differ in oxidation potential and ability to reoxidize glucose oxidase, and a siloxane copolymer; (2) construction of carbon paste electrodes containing glucose oxidase and each of these new polymeric relay systems; (3) electrochemical testing of these biosensors using cyclic voltammetry and a constant potential procedure. Emphasis will be placed on determining the optimal electron transfer relay system based on the following considerations: sensitivity to glucose, low operating potential, insensitivity to oxygen, and long-term stability.