RELATION OF OUTER CAPSID PROTEIN VP4 OF HUMAN AND ANIMAL ROTAVIRUSES

人和动物轮状病毒外衣壳蛋白VP4的关系

• 批准号: 3814264
• 负责人: M GORZIGLIA
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3814264
• 关键词: Rotavirus; antibody neutralization test; gene deletion mutation; gene expression; genetic mapping; genetic strain; nucleic acid sequence; protein sequence; serotyping; simian virus; virulence; virus envelope; virus genetics; virus protein

By primer extension, we determined the complete nucleotide sequence of the gene encoding the outer capsid protein VP4 of human rotavirus strain K8. The VP4 gene is 2,359 base pairs in length with 5'- and 3'-noncoding regions of 9 and 25 nucleotides, respectively. The gene contains a long open reading frame of 2,325 bases capable of coding for a protein of 775 amino acids. When compared to other human rotaviruses, the VP4 of the strain K8 had an insertion of one amino acid after the l35th residue as found in simian rotavirus strains, and, in addition, it had a deletion of one amino acid (575th residue). The VP4 gene of K8 was only 60-70% homologous in amino acid sequence to the VP4 of other virulent human rotaviruses. This is an unusual observation since the VP4s of all other virulent human rotavirus strains exhibit greater than 90% amino acid homology. In contrast, the VP7 amino acid sequence of the K8 strain was quite similar to that of other serotype 1 human rotaviruses (greater than 98% homology). In our previous study in which the cross-reactive neutralization epitopes on VP4 of human rotaviruses were analyzed, K8 was found to bear unique VP4 neutralization epitopes. This strain, which belongs to subgroup II and serotype 1, was not neutralized by any of six anti-VP4 neutralizing monoclonal antibodies which reacted with serotype 1, 3 and 4, or serotype 1 through 4 human rotavirus strains. Thus, the K8 strain appears to have a unique VP4 gene previously not described.

通过引物延伸，我们确定了完整的核苷酸序列， 编码人轮状病毒株K8的外衣壳蛋白VP 4的基因。 VP 4基因全长2，359个碱基对，5 '-和3'-非编码区 分别为9和25个核苷酸的区域。该基因含有一个长 一个2，325个碱基的开放阅读框架，能够编码775个碱基的蛋白质 个氨基酸与其他人轮状病毒相比， K8菌株在第135位残基后插入一个氨基酸， 在猴轮状病毒株中发现，此外，它有一个缺失， 一个氨基酸（第575位残基）。K8的VP 4基因仅占60-70%， 在氨基酸序列上与其它强毒人的VP 4同源 轮状病毒这是一个不寻常的观察，因为所有其他的VP 4 强毒人轮状病毒株显示大于90%的氨基酸 同源性相反，K8毒株的VP 7氨基酸序列是 与其他血清1型人轮状病毒非常相似（大于 98%同源性）。 在我们先前的研究中，交叉反应中和表位 对人轮状病毒VP 4基因进行了分析，发现K8具有独特的VP 4基因 中和表位该菌株属于亚组II， 血清型1，不被六种抗VP 4中和抗体中的任何一种中和 与血清型1、3和4或血清型1反应的单克隆抗体 通过4种人类轮状病毒株。因此，K8菌株似乎具有 独特的VP 4基因以前没有描述过。