MONOCLONAL ANTIBODIES DIRECTED TO VP8 SUBUNIT OF VP4

以前，抗重组轮状病毒VP 4的抗血清 目的：研究人VP 4蛋白的抗原多态性， 轮状病毒株。 VP 4的三种不同血清型和一种亚型 在17株人轮状病毒株中鉴定了一种蛋白， 代表五种不同的VP 7血清型。 用人VP 4的VP 8切割亚基免疫小鼠 轮状病毒KU、DS-1、1076或K8株诱导的抗体， 中和了VP 4亚基所来源的轮状病毒。 在 一个与接种VP 8-DS-1的小鼠的脾融合，14 通过ELISA检测与轮状病毒反应的杂交瘤。 一些 mAb仅识别同源的人轮状病毒DS-1株，而 其他的是交叉反应。 这些结果表明，VP 8亚基 VP 4的基因组含有特异性区域，可用于区分 人轮状病毒中的VP 4血清型。

Previously, antisera against recombinant outer capsid rotavirus VP4 proteins were used to study the VP4 antigenic polymorphism among human rotavirus strains. Three distinct serotypes and one subtype of the VP4 protein were identified among 17 human rotavirus strains that contained representatives of five different VP7 serotypes. Immunization of mice with the VP8 cleavage subunit of VP4 of the human rotavirus KU, DS-1, 1076, or K8 strain induced antibodies that neutralized the rotavirus from which the VP4 subunit was derived. In one fusion with the spleen of a mouse inoculated with VP8-DS-1, 14 hybridomas were detected that reacted with rotavirus by ELISA. Some mAbs only recognized the homologous human rotavirus DS-1 strain, whereas others were cross-reactive. These results suggest that the VP8 subunit of VP4 contains specific regions which can be useful in distinguishing VP4 serotypes among human rotaviruses.