RELATION OF OUTER CAPSID PROTEIN VP3 OF HUMAN AND ANIMAL ROTAVIRUSES

猴SA11、牛NCDV、猪OSU和OSU的VP3基因 猪轮状病毒戈特弗里德株的序列研究 分析。两株SA11株VP3的氨基酸同源性分析 菌株的VP3蛋白仅为82.7%，而其中一株 SA11株与NCDV的同源性为98.4%，提示 这个SA11VP3是由于不经意间的基因重组而产生的 另一家实验室被牛轮状病毒污染。 OSU与其他动物的VP3氨基酸同源性 轮状病毒约占80%。令人惊讶的是，VP3蛋白 戈特弗里德病毒与人类VP3密切相关 无症状轮状病毒株以各种方式出现，包括(1) 核苷酸序列和(2)高免抗血清的中和作用 或VP3交叉反应性中和单抗。 两株沙门氏菌VP3氨基酸序列的比较 NCDV强毒株和弱毒疫苗株(RIT 4237)，只发现了5个氨基酸替换，它们是 分散在整个蛋白质中，但不涉及胰酶 裂解位点。其中一株强毒株和减毒株 菌株只有一种氨基酸(残基76)不同，这表明 VP3以外的一个基因或一系列基因持续突变 它们对候选RIT疫苗的减毒负责 NCDV毒株。

The VP3 genes of the simian SA11, bovine NCDV, porcine OSU, and porcine Gottfried strains of rotavirus were studied by sequence analysis. The overall amino acid homology of the VP3 of two SA11 strains was only 82.7%; however the VP3 protein of one of these SA11 strains exhibited an homology of 98.4% with NCDV, suggesting that this SA11 VP3 was derived by inadvertent gene reassortment in another laboratory during contamination with a bovine rotavirus. The VP3 amino acid amino acid homology of OSU and other animal rotaviruses is approximately 80%. Surprisingly, the VP3 protein of Gottfried virus is closely related to the VP3 of human asymptomatic rotavirus strains in various ways including (1) nucleotide sequence and (2) neutralization by hyperimmune antisera or VP3 cross-reactive neutralizing monoclonal antibodies. A comparison of the deduced amino acid sequence of the VP3 of two virulent NCDV strains and an attenuated NCDV vaccine strain (RIT 4237), revealed only five amino acid substitutions which were scattered throughout the protein but did not involve the trypsin cleavage sites. One of the virulent strains and the attenuated strain differed in only one amino acid (residue 76) suggesting that a gene or a series of genes other than the VP3 sustained mutations which were responsible for attenuation of the candidate RIT vaccine strain of NCDV.