RELATION OF OUTER CAPSID PROTEIN VP3 OF HUMAN AND ANIMAL ROTAVIRUSES

人和动物轮状病毒外衣壳蛋白VP3的关系

• 批准号: 3818241
• 负责人: M GORZIGLIA
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3818241
• 关键词: Reoviridae disease; Rotavirus; antiserum; gene expression; gene mutation; genetic manipulation; genetic mapping; genetic strain; glycoproteins; monoclonal antibody; nucleic acid sequence; protein sequence; virulence; virus genetics; virus protein

The VP3 genes of the simian SA11, bovine NCDV, porcine OSU, and porcine Gottfried strains of rotavirus were studied by sequence analysis. The overall amino acid homology of the VP3 of two SA11 strains was only 82.7%; however the VP3 protein of one of these SA11 strains exhibited an homology of 98.4% with NCDV, suggesting that this SA11 VP3 was derived by inadvertent gene reassortment in another laboratory during contamination with a bovine rotavirus. The VP3 amino acid amino acid homology of OSU and other animal rotaviruses is approximately 80%. Surprisingly, the VP3 protein of Gottfried virus is closely related to the VP3 of human asymptomatic rotavirus strains in various ways including (1) nucleotide sequence and (2) neutralization by hyperimmune antisera or VP3 cross-reactive neutralizing monoclonal antibodies. A comparison of the deduced amino acid sequence of the VP3 of two virulent NCDV strains and an attenuated NCDV vaccine strain (RIT 4237), revealed only five amino acid substitutions which were scattered throughout the protein but did not involve the trypsin cleavage sites. One of the virulent strains and the attenuated strain differed in only one amino acid (residue 76) suggesting that a gene or a series of genes other than the VP3 sustained mutations which were responsible for attenuation of the candidate RIT vaccine strain of NCDV.

猴SA11、牛NCDV、猪OSU和 对猪Gottfried轮状病毒株进行了序列测定 分析. 两个SA11的VP3的总体氨基酸同源性 其中一株的VP3蛋白表达阳性率为82.7%， SA11株与NCDV的同源性为98.4%，表明SA11株与NCDV的同源性较高， 这种SA11 VP3是由于在 另一个实验室被牛轮状病毒污染。 OSU与其它动物的VP3氨基酸同源性 轮状病毒约占80%。 令人惊讶的是，VP3蛋白 Gottfried病毒的VP3基因与人的VP3基因密切相关 无症状轮状病毒株的各种方式，包括（1） 核苷酸序列和（2）超免疫抗血清的中和作用 或VP3交叉反应性中和单克隆抗体。 两株猪肺炎支原体VP3基因推导的氨基酸序列的比较 强毒NCDV毒株和减毒NCDV疫苗株（RIT 4237），仅揭示了5个氨基酸替换， 分散在蛋白质中，但不涉及胰蛋白酶 裂解位点 其中一种强毒株和减毒株 菌株仅在一个氨基酸（残基76）上不同，表明 VP3以外的一个或一系列基因持续突变 其负责候选RIT疫苗的减毒 NCDV毒株。