SEQUENCE OF THE SIXTH ROTAVIRUS GENE

编码猪瘟病毒VP6的第六个基因的全序列 两个I亚组动物轮状病毒(马尾猴轮状病毒SA-11和 牛轮状病毒Rf)和一个亚组II人轮状病毒(WA) 先前确定的。推导出的氨基酸序列比较 序列显示高度同源性；超过90%的 氨基酸在亚组I和亚组II之间是保守的 轮状病毒。 在本研究中，我们推导了VP6的氨基酸序列 另外4种轮状病毒株的蛋白质，每种都有不同的 亚群特异性：人轮状病毒1076株(亚群I)， 猪轮状病毒戈特化(亚群(II)，马轮状病毒 染色H-2(既不是亚群I也不是亚群II)和马轮状病毒株 FI-14(亚组I和亚组II)。这使我们能够比较VP6 轮状病毒双亚群或未知亚群的氨基酸序列 与属于亚组I或II的轮状病毒的反应性。 采用这种方法是为了试图识别表位。 负责亚组专一性。此外，重要的是 VP6蛋白亚群的三聚体构象 对表位进行了研究。 不同菌株的大多数VP6基因都含有一个独特的AUG。 相反，H-2毒株的VP6基因包含一个额外的AUG 在残数132-134，这导致第二个 VP6蛋白。两种VP6产物均呈三聚体构象 成熟的病毒粒子。

The complete nucleotide sequence of the sixth gene encoding VP6 of two subgroup I animal rotavirus (vervet monkey rotavirus SA-11 and bovine rotavirus RF) and one subgroup II human rotavirus (Wa) was determined previously. Comparison of the deduced amino acid sequences indicated a high degree of homology; more than 90% of the amino acids were conserved between subgroup I and subgroup II rotaviruses. In the present study we deduced the amino acid sequence of the VP6 protein of 4 additional rotavirus stains, each with a different subgroup specificity: human rotavirus strain 1076 (subgroup I), porcine rotavirus strain Gottfied (subgroup (II), equine rotavirus stained H-2 (neither subgroup I nor II) and equine rotavirus strain FI-14 (both subgroup I and II). This allowed us to compare the VP6 amino acid sequences of rotaviruses with dual or no known subgroup reactivity with those of rotaviruses belonging to subgroup I or II. This approach was taken in an attempt to identify epitopes responsible for subgroup specificity. In addition, the importance of the trimeric conformation of the VP6 protein to subgroup epitopes was investigated. Most of the VP6 genes the different strains contain a unique AUG. In contrast, the VP6 gene of H-2 strain contains an additional AUG at residue 132-134, which results in the translation of a second VP6 protein. Both VP6 products assume a trimeric conformation in the mature virion.