DEVELOPMENT OF RECOMBINANT BCG-VP8 VACCINES
重组 BCG-VP8 疫苗的开发
基本信息
- 批准号:3803272
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:
- 资助国家:美国
- 起止时间:至
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
Rotavizuses are the major cause of severe diarrhea in infants and young
children in both developed and developing countries. Two outer capsid
rotavirus proteins, VP7 and VP4, are associated with the induction of
neutralizing antibodies which have been shown in experimental animals to
be associated with resistance to illness. Serotype specificity is
associated primarily with VP7. However, recent studies have elucidated
the antigenic relationships of the VP4 of human rotaviruses which appear
to be less polymorphic than VP7. Human rotavirus strains that are
associated with symptomatic infection and that exhibit VP7 specificity
of serotype 1, 2, 3, 4 or 9 each possess a similar VP4 as determined by
neutralization assay. Objectives of this study were: (i) to use the VP8
subunit of VP4 to develop a BCG-VP8 recombinant and evaluate its ability
to induce specific rotavirus neutralizing antibodies in experimental
animals, and (ii) to utilize the information thus obtained for the
development of a human rotavirus vaccine for oral administration.
The cDNA representing the VP8 subunit of outer capsid rotavirus protein
VP4 from the human rotavirus KU strain was cloned in three different BCG
vectors. The VP8 subunit expressed in these systems was recognized in
an immunoblot assay by antibodies present in hyperimmune antiserum to:
(i) human rotavirus strain Wa, (ii) expressed KU-VP4, and (iii)
expressed KU-VP8, a cleavage subunit of VP4. These observations suggest
that the VP8 subunit was expressed in an antigenically correct form by
the recombinant as authentic VP8.
轮状病毒是婴幼儿严重腹泻的主要原因。
发达国家和发展中国家的儿童。两个外壳
轮状病毒蛋白,VP7和VP4,与诱导
已在实验动物中显示的中和抗体
与抵抗疾病联系在一起。血清型特异性是
主要与VP7有关。然而,最近的研究阐明了
人轮状病毒VP4的抗原性关系
比VP7基因更少的多态。人类轮状病毒株是
与症状性感染相关,并表现出VP7特异性
血清1、2、3、4或9的每一个都具有相似的VP4,由
中和试验。本研究的目的是:(I)使用VP8
VP4亚基构建卡介苗-VP8重组体及其活性评价
诱导特异性轮状病毒中和抗体的实验研究
动物,以及(Ii)利用这样获得的信息
口服人轮状病毒疫苗的研制。
代表轮状病毒外衣壳蛋白VP8亚基的cDNA
人轮状病毒KU株VP4基因在三种不同卡介苗中的克隆
向量。在这些系统中表达的VP8亚单位在
用高免抗血清中存在的抗体进行免疫印迹分析:
(I)人轮状病毒Wa株,(Ii)表达KU-VP4,和(Iii)
表达了VP4裂解亚基KU-VP8。这些观察结果表明
VP8亚单位以抗原性正确的形式通过
重组体为正品VP8。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('M GORZIGLIA', 18)}}的其他基金
RELATION OF OUTER CAPSID PROTEIN VP4 OF HUMAN AND ANIMAL ROTAVIRUSES
人和动物轮状病毒外衣壳蛋白VP4的关系
- 批准号:
3814264 - 财政年份:
- 资助金额:
-- - 项目类别:
SEQUENCE OF THE FOURTH ROTAVIRUS GENE AND ITS ROLE IN VIRULENCE
第四种轮状病毒基因的序列及其在毒力中的作用
- 批准号:
3822085 - 财政年份:
- 资助金额:
-- - 项目类别:
RELATION OF OUTER CAPSID PROTEIN VP3 OF HUMAN AND ANIMAL ROTAVIRUSES
人和动物轮状病毒外衣壳蛋白VP3的关系
- 批准号:
3818241 - 财政年份:
- 资助金额:
-- - 项目类别:
EXPRESSION AND RECOVERY OF RECOMBINANT ROTAVIRUS VP4 FROM INSECT CELL CULTURES
从昆虫细胞培养物中表达和回收重组轮状病毒 VP4
- 批准号:
3803263 - 财政年份:
- 资助金额:
-- - 项目类别:
EXPRESSION AND RECOVERY OF RECOMBINANT ROTAVIRUS VP4 FROM INSECT CELL CULTURES
从昆虫细胞培养物中表达和回收重组轮状病毒 VP4
- 批准号:
3809744 - 财政年份:
- 资助金额:
-- - 项目类别:
EXPRESSION OF ROTAVIRUS OUTER CAPSID PROTEIN VP4 BY RECOMBINANT ADENOVIRUS
重组腺病毒表达轮状病毒外衣壳蛋白 VP4
- 批准号:
3790838 - 财政年份:
- 资助金额:
-- - 项目类别:
THE VP6 GENE OF EQUINE ROTAVIRUS STRAIN H-2 ENCODES TWO VP6 PROTEINS
马轮状病毒H-2株的VP6基因编码两种VP6蛋白
- 批准号:
3814282 - 财政年份:
- 资助金额:
-- - 项目类别:
SYNTHETIC ANALOGS OF ROTAVIRUS GENOMIC RNA EXPRESSING A FOREIGN MARKER GENE
表达外源标记基因的轮状病毒基因组 RNA 的合成类似物
- 批准号:
3790873 - 财政年份:
- 资助金额:
-- - 项目类别:
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