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EXPRESSION AND RECOVERY OF RECOMBINANT ROTAVIRUS VP4 FROM INSECT CELL CULTURES

EXPRESSION AND RECOVERY OF RECOMBINANT ROTAVIRUS VP4 FROM INSECT CELL CULTURES
从昆虫细胞培养物中表达和回收重组轮状病毒 VP4
批准号：
3803263
负责人：
M GORZIGLIA
金额：
$0.0万
依托单位：
NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3803263
关键词：
DNA virus Insecta Rotavirus gene expression genetic manipulation insect virus microorganism mass culture protein degradation protein purification tissue /cell culture transfection virus antigen virus envelope virus protein

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英文摘要
The possibility of producing and purifying large quantities of recombinant rotavirus VP4 from insect cell cultures was investigated. Spodoptera frugiperda (Sf9) cells were grown in large spinner flasks and infected with a baculovirus expressing the rotavirus OSU VP4 gene. The expressed VP4 subsequently was purified from such cultures to determine the yield of recombinant protein. Conditions for growth of cells and attainment of a high yield of VP4 were optimized to improve the expression and recovery of VP4. The fate of the expressed VP4 antigen and its degradation were examined. Also, a practical purification scheme was developed to maximize the yield of VP4 from insect cell cultures and to reduce cost and complexity of a potential industrial- scale purification process.
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