STUDY OF GENOMIC REARRANGEMENT IN ROTAVIRUSES

轮状病毒基因组重排的研究

• 批准号: 3814283
• 负责人: M GORZIGLIA
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3814283
• 关键词: Rotavirus; double stranded RNA; gel electrophoresis; gene rearrangement; genetic strain; laboratory rabbit; messenger RNA; nucleic acid sequence; virus RNA; virus antigen; virus genetics

The complete nucleotide sequence of genomic double stranded RNA (dsRNA) segment 11, of the Alabama strain of rabbit rotavirus, was determined by sequencing dsRNA and mRNA using the dideoxy chain termination method. This strain has a "super short" RNA electrophoresis pattern. Its segment 11 is 1036 base pairs in length, 369 nucleotides longer than that reported for rotavirus strains with a "long" electropherotype. Sequence analysis indicated that the shift in genome segment 11 of Alabama strain to a "super short" RNA pattern (i.e., decreased mobility during electrophoresis) results from a simple duplication followed by a simple deletion. Two potential open reading frames (orf) frames are present. The first orf, 594 base pairs in length (encoding 198 amino acids), extends from nucleotide 22 to a stop codon (TAA) at nucleotides 616 to 618. A stretch of 92 nucleotides (from positions 619-711) separate the end of the first and the beginning of the second orf. Sixty-five of these nucleotides (from bases 625 to 690) are similar in sequence to the nucleotides from bases 169 to 234. In addition, 18 nucleotides from positions 691 to 708 are similar in sequence to those at positions 322 to 339. The second orf of gene 11 of Alabama strain extends from nucleotides 712 to 984 and is 272 base pairs in length. Of interest, this orf is a duplication of the sequence corresponding to nucleotides 349 to 615 and is followed by a 3' noncoding region of 52 nucleotides. This second orf, unique to the gene 11 of the Alabama strain, is capable of coding for a protein of 91 amino acids.

基因组双链RNA(DsRNA)全序列 兔轮状病毒阿拉巴马州株的第11片段被确定为 用双脱氧链终止法测定dsRNA和mRNA的序列。这 菌株有一种“超短”的RNA电泳谱。它的第11部分是 1036个碱基对，比报道的长369个核苷酸 具有“长”电泳型的轮状病毒株。序列分析 这表明阿拉巴马州毒株基因组第11段的转变是 短“RNA模式(即在电泳过程中迁移率降低) 由简单复制和简单删除所产生的结果。 存在两个潜在的开放阅读框架(ORF)框架。第一个兽人， 全长594个碱基对(编码198个氨基酸)，延伸自 在核苷酸616至618处的22个核苷酸到一个终止密码子(TAA)。一段延伸的 92个核苷酸(来自619-711位)分隔第一个和 第二个兽人的开始。其中65个核苷酸(来自 碱基625至690)在序列上与来自碱基169的核苷酸相似 到234。此外，691-708位的18个核苷酸也是相似的 按顺序排列到位置322至339处。基因11的第二个ORF 阿拉巴马州毒株从712位核苷酸延伸到984位核苷酸，全长272个碱基对 长度。有趣的是，这个ORF是序列的复制 对应于核苷酸349到615，后面跟着3‘非编码 52个核苷酸的区域。这是第二个orf，是该病毒基因11所独有的 阿拉巴马州株，能够编码91个氨基酸的蛋白质。