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EXPRESSION AND RECOVERY OF RECOMBINANT ROTAVIRUS VP4 FROM INSECT CELL CULTURES

EXPRESSION AND RECOVERY OF RECOMBINANT ROTAVIRUS VP4 FROM INSECT CELL CULTURES
从昆虫细胞培养物中表达和回收重组轮状病毒 VP4
批准号：
3809744
负责人：
M GORZIGLIA
金额：
$0.0万
依托单位：
NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3809744
关键词：
Insecta Rotavirus genetic manipulation insect virus protein purification tissue /cell culture virus protein

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中文摘要
翻译
生产和纯化大量重组蛋白的可能性 研究了来自昆虫细胞培养物的轮状病毒VP4。夜蛾 在大的转瓶中培养草地贪夜蛾（Sf9）细胞，并感染 表达轮状病毒OSU VP4基因的杆状病毒。表达的VP4 随后从这样的培养物中纯化，以确定 重组蛋白细胞生长和VP4高产率的条件 优化以提高VP4的表达。此外，一个实用的 本研究建立了一种从昆虫中最大限度地获得VP4的纯化方案 细胞培养和降低成本和复杂性的潜力 工业规模的纯化工艺。
英文摘要
The possibility of producing and purifying large quantities of recombinant rotavirus VP4 from insect cell cultures was investigated. Spodoptera frugiperda (Sf9) cells were grown in large spinner flasks and infected with a baculovirus expressing the rotavirus OSU VP4 gene. The expressed VP4 subsequently was purified from such cultures to determine the yield of recombinant protein. Conditions for growth of cells and high yield of VP4 were optimized to improve the expression of VP4. Also, a practical purification scheme was developed to maximize the yield of VP4 from insect cell cultures and to reduce cost and complexity of a potential industrial-scale purification process.
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会议论文
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RELATION OF OUTER CAPSID PROTEIN VP4 OF HUMAN AND ANIMAL ROTAVIRUSES
THE VP6 GENE OF EQUINE ROTAVIRUS STRAIN H-2 ENCODES TWO VP6 PROTEINS
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