MONOCLONAL ANTIBODIES DIRECTED TO VP8 SUBUNIT OF VP4

以前，抗重组轮状病毒VP4的抗血清 目的：研究人VP4蛋白的抗原多态性， 轮状病毒株。 VP4的三种不同血清型和一种亚型 在17株人轮状病毒株中鉴定了一种蛋白， 代表五种不同的VP7血清型。 结果来自cross- 用VP8抗血清进行免疫沉淀和中和试验表明 VP4的VP8切割亚基含有主要的抗原位点， 负责通过VP4对轮状病毒的轮状型特异性中和。 用人VP4的VP8切割亚基免疫小鼠 轮状病毒KU、DS-1、1076或K8株诱导的多克隆抗体， 中和了VP4亚基所来源的轮状病毒。 然而，在这方面， 迄今为止获得的大多数VP4单克隆抗体已经识别 异源菌株 我们将尝试生产特定类型的 使用由生产的“马赛克病毒”针对VP 4的单克隆抗体（MAbs） 用表达VP6的杆状病毒重组体共感染昆虫细胞， VP7和VP4基因。

Previously, antisera against recombinant outer capsid rotavirus VP4 proteins were used to study the VP4 antigenic polymorphism among human rotavirus strains. Three distinct serotypes and one subtype of the VP4 protein were identified among 17 human rotavirus strains that contained representatives of five different VP7 serotypes. Results from cross- immunoprecipitation and neutralization assays with VP8 antisera suggest that the VP8 cleavage subunit of VP4 contains the major antigenic sites responsible for the serotype-specific neutralization of rotavirus via VP4. Immunization of mice with the VP8 cleavage subunit of VP4 of the human rotavirus KU, DS-1, 1076, or K8 strain induced polyclonal antibodies that neutralized the rotavirus from which the VP4 subunit was derived. However, most of the VP4-monoclonal antibodies obtained so far have recognized heterologous strains. We will attempt to produce serotype-specific monoclonal antibodies (MAbs) against VP4 using "mosaic virus" produced by coinfection of insect cells with baculovirus recombinant expressing VP6, VP7, and VP4 genes.