GENETIC MAPPING OF MOUSE CHROMOSOMAL GENES

我们一直在分析体细胞杂种和种间（亚种）杂交 开发一个多基因座遗传图谱的小鼠主要包括 表达基因和假基因。 大多数基因都是通过分析绘制的 两组遗传杂交的后代，一个种间回交， 和亚种间回交 这些老鼠的DNA已经被分型 对于超过750个基因座，其中约一半已经预先定位， 允许将新定义的基因定位到 连锁图谱 这些研究导致了 数百个新基因，包括最近的脑cDNA， 编码骨形态发生蛋白、阿片受体蛋白、酪氨酸 激酶和磺基转移酶。 特定的地图位置可能很有用 由于与已知发育突变的接近性可以 鉴定这样的基因作为异常表型的潜在候选者。 因此，一个新的软骨衍生的形态发生因子的基因被定位 2号染色体上靠近突变bp的位点（短足症）。 随后的分析表明，这种突变是由于一种特定的 这一点的异常。 其他研究集中在 哺乳动物基因组中多基因家族的组织和 人和小鼠同源基因的比较连锁关系。

We have been analyzing somatic cell hybrids and inter(sub)species crosses to develop a multilocus genetic map of the mouse largely comprised of expressed genes and pseudogenes. Most genes are mapped by the analysis of the progeny of two sets of genetic crosses, an interspecies backcross and an intersubspecies backcross. DNAs from these mice have been typed for over 750 loci about half of which have been previously positioned to permit mapping of newly defined genes to specific positions on the linkage map. These studies have resulted in the genetic mapping of several hundred new genes including, most recently, brain cDNAs, genes encoding bone morphogenetic proteins, opioid receptor proteins, tyrosine kinases, and sulfotransferases. Specific map locations can be useful information since proximity to a known developmental mutation can identify such a gene as a potential candidate for the abnormal phenotype. Thus, a gene for a new cartilage-derived morphogenetic factor was mapped to a site on Chromosome 2 near the mutation bp (brachypodism). Subsequent analysis demonstrated that the mutation was due to a specific abnormality of this locus. Other studies have focussed on the organization of multigene families in the mammalian genome and on the comparative linkage relationships of homologous genes in man and mouse.