GENETIC MAPPING OF MOUSE CHROMOSOMAL GENES

我们一直在分析体细胞杂交和亚种间杂交。 开发小鼠的多基因座遗传图谱，主要包括 表达基因和假基因。大多数基因都通过分析得到了图谱 两组遗传杂交的后代，种间回交 和亚种间回交。这些小鼠的DNA已经被分型了 对于超过750个基因座，其中约一半以前被定位到 允许将新定义的基因映射到 连锁图。这些研究已经导致了基因图谱的绘制 数百个新基因，包括最近的大脑cDNA、基因 编码骨形态发生蛋白、阿片受体蛋白、酪氨酸 激动酶和磺基转移酶。特定的地图位置可能很有用 因为接近已知的发育突变的信息可以 确定这样一个基因是异常表型的潜在候选基因。 因此，一种新的软骨源性形态发生因子的基因被定位。 到2号染色体上突变BP(短足畸形)附近的一个位置。 随后的分析表明，突变是由于一种特定的 这个位置的异常。其他研究主要集中在 哺乳动物基因组中多基因家族的组织和在 人类和小鼠同源基因的比较连锁关系。

We have been analyzing somatic cell hybrids and inter(sub)species crosses to develop a multilocus genetic map of the mouse largely comprised of expressed genes and pseudogenes. Most genes are mapped by the analysis of the progeny of two sets of genetic crosses, an interspecies backcross and an intersubspecies backcross. DNAs from these mice have been typed for over 750 loci about half of which have been previously positioned to permit mapping of newly defined genes to specific positions on the linkage map. These studies have resulted in the genetic mapping of several hundred new genes including, most recently, brain cDNAs, genes encoding bone morphogenetic proteins, opioid receptor proteins, tyrosine kinases, and sulfotransferases. Specific map locations can be useful information since proximity to a known developmental mutation can identify such a gene as a potential candidate for the abnormal phenotype. Thus, a gene for a new cartilage-derived morphogenetic factor was mapped to a site on Chromosome 2 near the mutation bp (brachypodism). Subsequent analysis demonstrated that the mutation was due to a specific abnormality of this locus. Other studies have focussed on the organization of multigene families in the mammalian genome and on the comparative linkage relationships of homologous genes in man and mouse.