GENETIC MAPPING OF MOUSE CHROMOSOMAL GENES

描述人类基因组特征的主要目标之一是 制作模式物种的高密度遗传连锁图， 就像老鼠一样。 我们的绘图工作依赖于两种不同的方法。 传统上，我们使用中国仓鼠x小鼠体细胞杂交， 将新发现的基因分配到特定的染色体上。 这些杂种 继续是有用的，以确定基因的数量确定的基因， 具有多个基因组拷贝的杂交探针，如热休克 蛋白质基因，或绘制控制物种特异性性状的基因 在组织培养中表达，如干扰素γ的辅助因子 受体的 最近，我们一直在使用Southern印迹技术绘制基因图谱， 分析了两个遗传杂交的后代，一个种间杂交和一个种间杂交。 亚种间回交 这些杂交后代的DNA 已对多种多态性参考基因座进行分型，以允许绘制 将未知标记定位到连锁图上的特定位置。 这些研究 已经导致了大量基因的染色体图谱 包括在神经组织中表达的各种基因，钙通道， 基因，囊性纤维化跨膜传导的小鼠同源物 调节基因和各种转录因子基因。 几 这些基因定位在已知的小鼠突变处或附近， 这些突变的潜在候选人。 因此，一个基因特别 在黑素细胞中表达的基因定位在染色体10上的银基因座附近， 另一个在大脑中特异表达的基因被定位在大脑中， 神经系统缺损突变，并显示在突变缺损中缺失 小鼠

One of the major goals of the effort to characterize the human genome is the production of high density genetic linkage maps of model species such as the mouse. Our mapping efforts rely on two different approaches. Traditionally we have used Chinese hamster x mouse somatic cell hybrids to assign newly identified genes to specific chromosomes. These hybrids continue to be useful to determine the number of genes identified by hybridization probes with multiple genomic copies, like the heat shock proteins genes, or to map genes which govern species specific traits expressed in tissue culture like the accessory factor for interferon gamma receptor. More recently, we have been mapping genes using Southern blot analysis of the progeny of two genetic crosses, an interspecies and an intersubspecies backcross. DNAs from the progeny of these crosses have been typed for a variety of polymorphic reference loci to permit mapping of unknown markers to specific positions on the linkage map. These studies have resulted in the chromosomal mapping of a large number of genes including the various genes expressed in nervous tissue, calcium channel genes, the mouse homolog of the cystic fibrosis transmembrane conductance regulator gene, and various genes for transcriptional factors. Several of these genes map at or near known mouse mutations and therefore are potential candidates for these mutations. Thus, one gene specifically expressed in melanocytes was mapped near the silver locus on chromosome 10, and another gene specifically expressed in brain was mapped near the coloboma neurological mutation and shown to be deleted in mutant coloboma mice.