CELLULAR GENES INVOLVED IN NEOPLASTIC TRANSFORMATION

小鼠逆转录病毒通过病毒传播的遗传学研究 小鼠生殖系、对外源性感染的易感性和病毒诱导 致癌基因的发现和染色体定位 这些现象涉及大量的遗传位点。关于体细胞的分析 细胞杂交体已经被单独或与经典的 孟德尔杂交定位以下基因：小鼠乳腺肿瘤前病毒 C3H/HeJ小鼠14号染色体和BALB/c和An小鼠6号染色体的基因座 11号染色体的内源性MuLV前病毒；3 3条染色体上的癌基因；肿瘤特异的生态型MuLV整合位点 7、15和19号染色体；肿瘤相关乳腺病毒整合 3个不同的染色体上；干扰素Beta-1基因 染色体4。杂交细胞也被用来分析差异 内源性小鼠白血病病毒基因在化学诱导中的表达 缺乏二次感染所需受体的杂交细胞。 生态型细胞表面受体的作用也已在 自身反应性胸腺细胞识别靶细胞。

Genetic studies on the transmission of murine retroviruses through the mouse germline, susceptibility to exogenous infection, and virus-induced oncogenis have led to the identification and chromosomal mapping of numerous genetic loci involved in these phenomena. The analysis of somatic cell hybrids has been used either alone or in conjunction with classical Mendelian crosses to map the following genes: mouse mammary tumor proviral loci to chromosome 14 in C3H/HeJ mice and to chromosome 6 in BALB/c and an endogenous MuLV provirus to chromosome 11; the cellular homologs of 3 oncogenes to 3 chromosomes; tumor-specific ecotropic MuLV integration sites to chromosomes 7, 15 and 19; tumor-associated mammary virus integration sites to 3 different chromosomes; and the interferon Beta-1 genes to chromosome 4. Hybrid cells have also been used to analyze the differential expression of endogenous murine leukemia viral loci in chemically induced hybrid cells which lack the receptors required for secondary infection. The role of the ecotropic cell surface receptor has also been examined in target cell recognition by autoreactive thymocytes.