CELLULAR GENES INVOLVED IN NEOPLASTIC TRANSFORMATION

参与肿瘤转化的细胞基因

• 批准号: 4688453
• 负责人: C A KOZAK
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4688453
• 关键词: cytogenetics; gene expression; genetic mapping; genetic recombination; genetic regulation; hybrid cells; interferons; mammary tumor virus; molecular biology; molecular cloning; murine leukemia virus; neoplasm /cancer genetics; nucleic acid sequence; oncogenes; provirus; structural genes; tissue /cell culture; viral leukemogenesis; virus genetics

Genetic studies on the transmission of murine retroviruses through the mouse germline, susceptibility to exogenous infection, and virus-induced oncogenis have led to the identification and chromosomal mapping of numerous genetic loci involved in these phenomena. The analysis of somatic cell hybrids has been used either alone or in conjunction with classical Mendelian crosses to map the following genes: mouse mammary tumor proviral loci to chromosome 14 in C3H/HeJ mice and to chromosome 6 in BALB/c and an endogenous MuLV provirus to chromosome 11; the cellular homologs of 3 oncogenes to 3 chromosomes; tumor-specific ecotropic MuLV integration sites to chromosomes 7, 15 and 19; tumor-associated mammary virus integration sites to 3 different chromosomes; and the interferon Beta-1 genes to chromosome 4. Hybrid cells have also been used to analyze the differential expression of endogenous murine leukemia viral loci in chemically induced hybrid cells which lack the receptors required for secondary infection. The role of the ecotropic cell surface receptor has also been examined in target cell recognition by autoreactive thymocytes.

鼠逆转录病毒通过肠道传播的遗传学研究 小鼠种系、对外源感染的易感性和病毒诱导的 致癌基因的鉴定和染色体作图 这些现象涉及许多遗传位点。 体细胞分析 细胞杂交已单独使用或与经典结合使用 孟德尔杂交绘制以下基因图谱：小鼠乳腺肿瘤原病毒 C3H/HeJ 小鼠中的 14 号染色体位点和 BALB/c 中的 6 号染色体位点 内源性 MuLV 原病毒至 11 号染色体； 3的细胞同系物 癌基因至3条染色体；肿瘤特异性亲向性 MuLV 整合位点 至 7、15 和 19 号染色体；肿瘤相关乳腺病毒整合 3 个不同染色体的位点；和干扰素 Beta-1 基因 4号染色体。杂交细胞也被用来分析差异 化学诱导的内源性小鼠白血病病毒位点的表达 缺乏继发感染所需受体的杂交细胞。 亲嗜性细胞表面受体的作用也已在 自身反应性胸腺细胞识别靶细胞。