GENETIC MAPPING OF MOUSE CHROMOSOMAL GENES

小鼠染色体基因的遗传图谱

• 批准号: 3746500
• 负责人: C A KOZAK
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3746500
• 关键词: animal breeding; animal genetic material tag; animal population genetics; cartilage disorder; cytogenetics; cytokine receptors; gene mutation; genetic mapping; genetic markers; genetic models; immunodeficiency; interleukin 2; laboratory mouse; laminin; linkage mapping; muscular dystrophy; mutant

We have been involved in an ongoing effort to develop a multilocus genetic map of the mouse. Most of this work has been accomplished by the analysis of the progeny of two genetic crosses, an interspecies backcross and an intersubspecies backcross. DNAs from these mice have been typed for a variety of reference loci to permit mapping of newly defined genes of unknown map location to specific positions on the linkage map. These studies have resulted in the genetic mapping of a large number of genes including, most recently, various genes expressed in nervous tissue, transcriptional regulators, protein kinases and factors involved in bone formation. Specific map locations can be useful information since proximity to a known developmental mutation can identify such a gene as a potential candidate for the abnormal phenotype. Thus, one gene encoding a major stuctural component of cartilage was mapped near the cmd (cartilage matrix deficiency) mutation, and cmd mice were shown to have a 7 bp deletion of this gene. In another example, the murine merosin gene was mapped near dy (dystrophia muscularis) and we identified a deficiency of merosin in mutant mice suggesting that a defect in this laminin gene is responsible for this disorder. Finally, the murine interleukin 2 gamma receptor chain gene (Il2rg) was mapped to a position on the X chromosome near the xid immunodeficiency mutation, although further investigation indicated that Il2rg was not responsible for xid.

我们一直致力于开发多位点 小鼠的遗传图谱。 大部分工作已由以下人员完成 对两个遗传杂交后代（种间杂交）的分析 回交和亚种间回交。 这些小鼠的 DNA 具有 已键入各种参考基因座，以允许新的映射 将未知图谱位置的基因定义为特定位置 联动图。这些研究已经绘制出一个基因图谱 大量基因，包括最近的各种基因 在神经组织、转录调节因子、蛋白质中表达 参与骨形成的激酶和因子。 具体地图 位置可能是有用的信息，因为靠近已知的地点 发育突变可以将这样的基因识别为潜在的 异常表型的候选者。 因此，一个基因编码一个主要的 软骨的结构成分被映射到 cmd 附近（软骨 基质缺陷）突变，cmd 小鼠显示有 7 bp 删除这个基因。 在另一个例子中，小鼠 merosin 基因 被绘制在 dy（肌营养不良）附近，我们发现了一个 突变小鼠中的 merosin 缺乏表明这种缺陷 层粘连蛋白基因是造成这种疾病的原因。 最后是鼠类 白介素 2 γ 受体链基因 (Il2rg) 被映射到 X 染色体上靠近 xid 免疫缺陷突变的位置， 尽管进一步调查表明 Il2rg 并非 负责xid。