GENETIC MAPPING OF MOUSE CHROMOSOMAL GENES

我们一直在努力开发一种多位点的 老鼠的基因图谱 这项工作的大部分已经完成， 对两个遗传杂交的后代进行分析， 回交和亚种间回交。 这些老鼠的DNA 已经为各种参考基因座分型，以允许对新的 将未知图谱位置的定义基因定位到 连锁图谱这些研究已经导致了一个基因图谱的产生。 大量的基因，包括最近的各种基因， 神经组织表达，转录调节因子，蛋白质 与骨形成有关的激酶和因子。 特定地图 位置可以是有用的信息，因为接近已知的 发育突变可以识别这样的基因作为一个潜在的 异常表型的候选者。 因此，一个基因编码一个主要的 软骨的结构成分被映射到CMD（软骨）附近 基质缺乏）突变，并且显示CMD小鼠具有7bp的 删除这个基因。 在另一个实例中，鼠裂体蛋白基因 被映射到dy（肌营养不良）附近，我们确定了一个 在突变小鼠中缺乏merosin，这表明这种缺陷 层粘连蛋白基因是导致这种疾病的原因。 最后，鼠 白细胞介素2 γ受体链基因（IL 2rg）定位于一个 X染色体上靠近xid免疫缺陷突变的位置， 尽管进一步的研究表明，Il2rg不是 负责XID。

We have been involved in an ongoing effort to develop a multilocus genetic map of the mouse. Most of this work has been accomplished by the analysis of the progeny of two genetic crosses, an interspecies backcross and an intersubspecies backcross. DNAs from these mice have been typed for a variety of reference loci to permit mapping of newly defined genes of unknown map location to specific positions on the linkage map. These studies have resulted in the genetic mapping of a large number of genes including, most recently, various genes expressed in nervous tissue, transcriptional regulators, protein kinases and factors involved in bone formation. Specific map locations can be useful information since proximity to a known developmental mutation can identify such a gene as a potential candidate for the abnormal phenotype. Thus, one gene encoding a major stuctural component of cartilage was mapped near the cmd (cartilage matrix deficiency) mutation, and cmd mice were shown to have a 7 bp deletion of this gene. In another example, the murine merosin gene was mapped near dy (dystrophia muscularis) and we identified a deficiency of merosin in mutant mice suggesting that a defect in this laminin gene is responsible for this disorder. Finally, the murine interleukin 2 gamma receptor chain gene (Il2rg) was mapped to a position on the X chromosome near the xid immunodeficiency mutation, although further investigation indicated that Il2rg was not responsible for xid.