GENETIC CONTROL OF RESISTANCE TO FRIEND VIRUS IN WILD MOUSE POPULATIONS

野生小鼠群体对朋友病毒抵抗力的基因控制

• 批准号: 4688452
• 负责人: C A KOZAK
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4688452
• 关键词: Friend virus; R factors; gene expression; genetic mapping; genetic regulation; genetic strain; microorganism immunology; molecular biology; molecular cloning; neoplasm /cancer genetics; neoplasm /cancer immunology; nucleic acid sequence; provirus; surface antigens; viral leukemogenesis; virus related neoplasm /cancer; virus replication

Studies on the genetics of viral leukemogenesis in wild mice have revealed that these mice differ substantially from the inbred strains. Analysis of a variety of feral mouse populations have shown that almost all lack ecotropic-MuLV related sequences. MuLVs with ecotropic host range have been isolated from M. hortulanus, M. m. castaneus and M. m. molossinus. Data from Southern blot hybridizations indicate that most of these viruses differ from ecotropic MuLVs of inbred mice in their hybridization properties and internal restriction maps. Cells of most wild mice also differ from inbred mice in their susceptibility to exogenous infection. Most lack the restriction defined by the known alleles at the Fv-1 locus. Genetic crosses demonstrated that 2 of these mice, M spretus and M. praetextus, carry the rare resistance allele at Fv-2. These mice also differ from laboratory mice in their susceptibility to xenotropic MuLVs. This trait is controlled by a single chromosome 1 locus, designated Sxv, which may represent a wild mouse polymorphism of the MCF-MuLV receptor locus.

对野生小鼠病毒性白血病发生的遗传学研究表明， 这些小鼠与近交系小鼠有很大不同 分析 各种野生小鼠种群表明，几乎所有的缺乏 嗜生态性MuLV相关序列。 具有亲嗜性寄主范围的MuLV具有 从M. hortulanus，M. M. castaneus和M. M. Molossinus。 Southern印迹杂交的数据表明，这些病毒中的大多数 与近交系小鼠的亲嗜性MuLV在杂交中不同 属性和内部限制图。 大多数野生小鼠的细胞也 与近交系小鼠不同的是它们对外源性感染的易感性。 大多数缺乏Fv-1基因座上已知等位基因所定义的限制。 遗传杂交表明，其中2只小鼠，M spretus和M。 praetextus在Fv-2处携带罕见的抗性等位基因。 这些小鼠还 与实验室小鼠不同的是它们对异嗜性MuLV的易感性。 这种性状由一个1号染色体基因座控制，命名为Sxv， 其可能代表MCF-MuLV受体的野生小鼠多态性 基因座