CONTROL OF RESISTANCE TO VIRAL LEUKOMOGENESIS IN WILD MOUSE POPULATIONS

控制野生小鼠群体对病毒性白血病发生的抗性

• 批准号: 3822030
• 负责人: C A KOZAK
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3822030
• 关键词: Retroviridae disease; animal population genetics; genetic mapping; genetic regulation; genetic strain; genetic transcription; molecular oncology; molecular pathology; murine leukemia virus; neoplasm /cancer genetics; nucleic acid sequence; oncogenes; oncogenic virus; pathology; viral leukemogenesis; virus genetics; virus infection mechanism; virus related neoplasm /cancer

The genetic basis of disease induction by murine leukemia viruses (MuLVs) has been studied largely by analysis of the different inbred strains of the laboratory mouse. However, wild mice show a greater genetic diversity than the inbred strains, and our use of these animals has expanded our understanding of viral leukemogenesis in several important ways. First, direct analysis of wild mice shows that they differ markedly from the inbred strains n the number and type of endogenous MuLVs they carry. We have now shown that xenotropic and MCF-related MuLV env genes are present as endogenous copies in different populations of wild mice. We have also shown that amphotropic MuLVs are transmitted as exogenous virus in Southern California mice and are not present as germline copies in any mammalian DNA tested. Second, infectious viruses from wild mice differ from their laboratory mouse counterparts at the molecular level and have different patterns of pathogenesis. Transcription of endogenous MuLV sequences has been examined in various mild mice, and a new MuLV isolate, HoMuLV, has been shown to be leukemogenic in inbred strains.

小鼠白血病病毒致病的遗传学基础 （MuLVs）主要通过分析不同的 实验室小鼠的近交系。 然而，野生老鼠显示， 更大的遗传多样性比近交系，我们使用的 这些动物扩展了我们对病毒的理解 在几个重要的方面影响白血病的发生。 第一，直接分析 野生小鼠的研究表明，它们与近交系小鼠有明显的不同， 它们携带的内源性MuLV的数量和类型。 我们现在已经证明，异嗜性和MCF相关的MuLV env 基因作为内源性拷贝存在于不同群体中， 野生老鼠 我们还表明，嗜热性MuLV是 在南加州小鼠中作为外源性病毒传播， 在任何检测的哺乳动物DNA中均不存在生殖系拷贝。 第二，来自野生小鼠的传染性病毒与它们的 实验室小鼠在分子水平上的对应物， 不同的发病模式。 内源性转录 MuLV序列已经在各种轻度小鼠中进行了检查， 一种新的MuLV分离株HoMuLV已被证明具有致白血病性 在近交系中。