IMMUNOLOGIC STUDIES ON SCHISTOSOMIASIS

这项研究计划的目的是研究 日本血吸虫病的保护性免疫和免疫病理学研究 免疫干预的终极目标。在以下方面取得了进展 年内有以下几个方面。 A)使用IL-12预防虫卵肉芽肿的形成。细胞因子 IL-12被证明在给药时可阻断肺部虫卵病理 在肉芽肿形成的同时进行预防性免疫 当与鸡蛋免疫一起使用时，可对抗病理。 B)上调和下调细胞因子对疫苗的影响-- 诱导免疫。在接种疫苗时给予IL-12 辐射后的尾蚴显示出显著增强保护作用。 豁免权。相反，接种P菌株的免疫力下降 用死亡抗原免疫小鼠和C57BL/6小鼠 肌肉内的或静脉内的，与保护性的皮内相反 路线被证明与生产 下调细胞因子IL-4、IL-10和转化生长因子-β。 C)细胞因子激活的人杀灭血吸虫的机制 巨噬细胞和内皮细胞。细胞因子激活的人 巨噬细胞通过一种新的氮气杀死血吸虫 氧、呼吸爆发的非独立机制。

The aim of this research program is to investigate mechanisms of protective immunity and immunopathology in schistosomiasis with the ultimate goal of immunologic intervention. Progress was achieved in the following areas during the year. A) Prevention of egg granuloma formation with IL-12. The cytokine IL-12 was shown to block pulmonary egg pathology when given at the same time as granuloma formation and to prophylactically immunize against pathology when used in conjunction with egg immunization. B) Effects of upregulatory and downregulatory cytokines on vaccine- induced immunity. IL-12 when given at the time of vaccination with irradiated cercariae was shown to markedly enhance protective immunity. Conversely, the reduced immunity of vaccinated P-strain mice and C57BL/6 mice immunized with dead antigen via the intramuscular or intravenous as opposed to protective intradermal routes was shown to be associated with the production of the downregulatory cytokines IL-4 and IL-10 and TGF-beta, respectively. C) Mechanism of killing of schistosomula by cytokine-activated human macrophages and endothelial cells. Cytokine activated human macrophages were shown to kill schistosomula via a novel nitrogen oxide, respiratory burst independent mechanism.