DEVELOPMENTAL ADAPTATIONS OF TRYPANOSOMA CRUZI TO THE VERTEBRATE IMMUNE SYSTEM

克氏锥虫对脊椎动物免疫系统的发育适应

• 批准号: 3818149
• 负责人: A SHER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3818149
• 关键词: Trypanosoma cruzi; affinity chromatography; complement; complement receptor; complementary DNA; developmental genetics; gene expression; genetic translation; host organism interaction; immunogenetics; intracellular parasitism; laboratory mouse; lectin; macrophage; membrane proteins; microorganism culture; microorganism immunology; molecular cloning; protooncogene; protozoal antigen; surface antigens; tissue /cell culture; trypanosomiasis

In this project we have been studying developmental adaptations of T. cruzi to the vertebrate host and, in particular the molecular basis of adaptive changes occurring during the differentiation of epimastigotes (vector stage) to metacyclic trypomastigotes (infective stage). A. Studies on developmental control of intracellular survival in macrophages Epimastigotes were shown to enter mouse macrophages by CR3 receptors while the entry and survival of metacyclic trypomastigotes was shown to be enhanced by complement but also to depend on fibronectin receptors. B. Analysis of stage-specific gene expression in metacyclic forms Metacyclic stage specific cDNA's were cloned from T. cruzi and L. major. Sequence analysis indicated that the principal T. cruzi clone has homology with a c-fos proto-oncogene while the L. major clone has homology with beta-chain of RNA polymerase.

在这个项目中，我们一直在研究 克氏锥虫对脊椎动物宿主，特别是分子 在分化过程中发生适应性变化的基础 上鞭毛体(介质期)到后环体鞭毛体 (感染期)。 A.细胞内存活的发育控制研究 巨噬细胞 CR3显示上胚体进入小鼠巨噬细胞 受体同时进入和存活亚环 研究表明，补体不仅能增强类鞭毛虫的功能，而且还能促进 依赖于纤维连接蛋白受体。 B.以偏环形式分析特定阶段的基因表达 克隆了克氏木霉和L. 少校。序列分析表明，主要的克氏锥虫 克隆与c-fos原癌基因有同源性，而L. 该克隆与RNA聚合酶的β链具有同源性。