DEVELOPMENTAL ADAPTATIONS OF TRYPANOSOMA CRUZI TO THE VERTEBRATE IMMUNE SYSTEM

在这个项目中，我们一直在研究 T. cruzi对脊椎动物宿主的作用，特别是 在分化过程中发生适应性变化的基础 上鞭毛体（媒介阶段）到后环锥鞭毛体 （感染期）。 A. 细胞内存活发育调控的研究 巨噬 CR 3显示外鞭毛体进入小鼠巨噬细胞 受体，而进入和生存的元环 锥虫鞭毛体被证明是增强补体，但也 依赖于纤维连接蛋白受体。 B。 后循环形式中阶段特异性基因表达的分析 从T. cruzi和L. 少校 序列分析表明，T. cruzi 克隆与c-fos原癌基因同源，而L.主要 克隆与RNA聚合酶β链具有同源性。

In this project we have been studying developmental adaptations of T. cruzi to the vertebrate host and, in particular the molecular basis of adaptive changes occurring during the differentiation of epimastigotes (vector stage) to metacyclic trypomastigotes (infective stage). A. Studies on developmental control of intracellular survival in macrophages Epimastigotes were shown to enter mouse macrophages by CR3 receptors while the entry and survival of metacyclic trypomastigotes was shown to be enhanced by complement but also to depend on fibronectin receptors. B. Analysis of stage-specific gene expression in metacyclic forms Metacyclic stage specific cDNA's were cloned from T. cruzi and L. major. Sequence analysis indicated that the principal T. cruzi clone has homology with a c-fos proto-oncogene while the L. major clone has homology with beta-chain of RNA polymerase.