DEVELOPMENTAL ADAPTATIONS OF TRYPANOSOMA CRUZI TO THE VERTEBRATE IMMUNE SYSTEM

在这个项目中，我们一直在研究发育适应 T. cruzi 对脊椎动物宿主，特别是分子 分化过程中发生适应性变化的基础 上鞭毛体（矢量阶段）到后循环锥鞭毛体 （感染阶段）。 A. 细胞内存活发育控制的研究 巨噬细胞 上鞭毛体被证明可以通过 CR3 进入小鼠巨噬细胞 受体，而后循环的进入和存活 锥鞭毛体被证明可以被补体增强，但也可以 依赖于纤连蛋白受体。 B. 后循环形式阶段特异性基因表达分析 从 T. cruzi 和 L. 克隆了后循环阶段特异性 cDNA。 专业。 序列分析表明主要 T. cruzi 克隆与 c-fos 原癌基因具有同源性，而 L. Major 克隆与RNA聚合酶的β链具有同源性。

In this project we have been studying developmental adaptations of T. cruzi to the vertebrate host and, in particular the molecular basis of adaptive changes occurring during the differentiation of epimastigotes (vector stage) to metacyclic trypomastigotes (infective stage). A. Studies on developmental control of intracellular survival in macrophages Epimastigotes were shown to enter mouse macrophages by CR3 receptors while the entry and survival of metacyclic trypomastigotes was shown to be enhanced by complement but also to depend on fibronectin receptors. B. Analysis of stage-specific gene expression in metacyclic forms Metacyclic stage specific cDNA's were cloned from T. cruzi and L. major. Sequence analysis indicated that the principal T. cruzi clone has homology with a c-fos proto-oncogene while the L. major clone has homology with beta-chain of RNA polymerase.