MOLECULAR STUDIES OF THE GENOME AND SURFACE OF SCHISTOSOMA MANSONI

曼索尼血吸虫基因组和表面的分子研究

• 批准号: 4688414
• 负责人: A SHER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4688414
• 关键词: antiantibody; bacteriophage lambda; complementary DNA; gene expression; genetic library; genetic manipulation; genome; immunological substance; molecular cloning; monoclonal antibody; surface antigens

Our progress this year consisted of a study of the dynamics of schistosome surface antigen expression in vivo and the cloning in an expression vector of several S. mansoni genes encoding antigens recognized by immune mice. A. Surface antigen modulation. While in vivo derived schistosomula express little if any serologically detectable surface antigen, schistosomula maturing in vitro retain their surface antigenicity. This difference was investigated and shown to be due to the rapid shedding of surface antigens when schistosomula are transferred from the in vitro to the in vivo environment. B. Antigen cloning studies. From a series of clones selected from a lambda gt-11 S. mansoni library, two clones were identified which encode antigens recognized by immune mouse antibodies. The antigenicity and immunogenecity of the fusion proteins produced by these clones were characterized.

我们今年的进展包括研究了 体内表面抗原表达和在表达载体中的克隆 几个S。mansoni基因编码免疫小鼠识别的抗原。 A.表面抗原调节。 而在体内衍生的胚泡表达 几乎没有血清学可检测到的表面抗原， 在体外成熟保留它们的表面抗原性。 该差异 研究表明，这是由于表面抗原的快速脱落 当囊胚从体外转移到体内时， 环境 B。抗原克隆研究。 从一系列的克隆中选择一个lambda gt-11 S. mansoni文库中，鉴定了两个编码抗原的克隆 被免疫小鼠抗体识别。 抗原性和免疫原性 对这些克隆产生的融合蛋白进行了表征。