MOLECULAR STUDIES OF THE GENOME AND SURFACE OF SCHISTOSOMA MANSONI

曼索尼血吸虫基因组和表面的分子研究

• 批准号: 3818150
• 负责人: A SHER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3818150
• 关键词: Pongidae; Schistosoma mansoni; anthelmintics; complement pathway; complementary DNA; gene expression; genetic library; genetic manipulation; genetic markers; genome; guinea pigs; helminthic antigen; host organism interaction; immunity; immunogenetics; membrane proteins; messenger RNA; microorganism immunology; molecular biology; molecular cloning; molecular genetics; nucleic acid sequence; parasitic disease chemotherapy; schistosomiasis; surface antigens

The aim of this project is to characterize schistosome surface molecules relevant to immunity, to analyze the genome of the parasite in relation to specific biological properties of the organism and to clone important schistosome immunogens. A. Molecular biology of paramyosin. Paramyosin was shown to be encoded by an unusually large transcript. cDNA's comprising unsequenced portions of the molecule were isolated along with peptides in these same regions. B. Demonstration of lipid anchors on schistosomulum membrane proteins. Four major schistosomulum surface antigens of Mr 70K, 32k-38k and 18k were shown to be anchored in the tegument by phosphatidylinositol linkages and released in the form of membrane vesicles. C. Detection of host DAF on the schistosome membrane. Using specific antibodies, host decay accelerating factor (DAF) was identified in the membrane of schistosomes recovered from monkeys and guinea pigs.

本项目的目的是表征血吸虫表面