IMMUNOLOGIC STUDIES ON SCHISTOSOMIASIS

这个项目的目的是研究免疫和免疫的机制 血吸虫病的免疫逃避，最终目标是 开发一种使用已定义抗原的实验性疫苗。 A.用纯化的副肌球蛋白和GST进行疫苗接种试验。 副肌球蛋白和谷胱甘肽-S转移酶(GST)均显示 卡介苗或皂苷诱导小鼠保护性免疫 佐剂，而两种抗原结合时没有相加作用。 B.流行人群对副肌球蛋白的识别。入院的病人 对巴西野外人群进行了抗体筛查 副肌球蛋白。无症状、未感染的患者被发现 出现比感染患者更高的反应，而肝脾 平均而言，患者的反应低于任何一组。 C.非副肌球蛋白T细胞保护性的特征 免疫原。非副肌球蛋白分子可以作为 用T细胞表征保护性免疫的免疫原 免疫印迹和层析技术。 D.抗Sm-200抗体在吡喹酮治疗中的作用。在……里面 被动转移研究抗200 kD的单抗 结果表明，结节糖蛋白可恢复U-T细胞的功能。 被抑制的小鼠在治疗时消除成虫 吡喹酮。

The aim of this project is to study mechanisms of immunity and immune evasion in schistosomiasis with the ultimate goal of developing an experimental vaccine employing defined antigens. A. Vaccination trials with purified paramyosin and GST. Paramyosin and glutathione-S transferase (GST) were both shown to induce protective immunity in mice using either BCG or saponin as adjuvants, while combining the two antigens had no additive effect. B. Recognition of paramyosin by endemic population. Patients in a Brazilian field population were screened for antibodies against paramyosin. Asymptomatic, non-infected individuals were found to develop higher responses than infected patients while hepatosplenic patients developed lower response on average than either group. C. Characterization of non-paramyosin T cell protective immunogens. Non-paramyosin molecules which could serve as immunogens for protective immunity were characterized using T cell immunoblotting and chromatographic techniques. D. Role of antibodies against Sm-200 in praziquantel therapy. In passive transfer studies a monoclonal antibody against a 200 kD tubercle glycoprotein was shown to restore the ability of u- suppressed mice to eliminate adult worms when treated with praziquantel.