IMMUNOLOGIC STUDIES ON SCHISTOSOMIASIS

血吸虫病的免疫学研究

• 批准号: 3818153
• 负责人: A SHER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3818153
• 关键词: Bacillus Calmette Guerin vaccine; Schistosoma mansoni; anthelmintics; athymic mouse; disease /disorder model; helminthic antigen; human therapy evaluation; human tissue; humoral immunity; immunological substance; immunopathology diagnosis; immunosuppression; immunotherapy; isoquinolines; laboratory mouse; major histocompatibility complex; microorganism immunology; monoclonal antibody; parasitic disease chemotherapy; schistosomiasis; vaccines

The aim of this project is to study mechanisms of immunity and immune evasion in schistosomiasis with the ultimate goal of developing an experimental vaccine employing defined antigens. A. Vaccination trials with purified paramyosin and GST. Paramyosin and glutathione-S transferase (GST) were both shown to induce protective immunity in mice using either BCG or saponin as adjuvants, while combining the two antigens had no additive effect. B. Recognition of paramyosin by endemic population. Patients in a Brazilian field population were screened for antibodies against paramyosin. Asymptomatic, non-infected individuals were found to develop higher responses than infected patients while hepatosplenic patients developed lower response on average than either group. C. Characterization of non-paramyosin T cell protective immunogens. Non-paramyosin molecules which could serve as immunogens for protective immunity were characterized using T cell immunoblotting and chromatographic techniques. D. Role of antibodies against Sm-200 in praziquantel therapy. In passive transfer studies a monoclonal antibody against a 200 kD tubercle glycoprotein was shown to restore the ability of u- suppressed mice to eliminate adult worms when treated with praziquantel.

该项目的目的是研究免疫机制， 血吸虫病的免疫逃避， 开发使用确定抗原的实验疫苗。 A. 用纯化的副肌球蛋白和GST进行疫苗接种试验。 副肌球蛋白和谷胱甘肽-S转移酶（GST）都被证明是 用BCG或皂苷诱导小鼠保护性免疫 佐剂，而组合两种抗原没有累加效应。 B。 地方性人群对副肌球蛋白的识别。 患者 对巴西野外人群进行了抗体筛查， 副肌球蛋白 无症状，未感染的个人被发现， 比感染患者产生更高的反应， 患者的平均反应低于两组。 C. 非副肌球蛋白T细胞保护性的表征 免疫原 非副肌球蛋白分子， 使用T细胞鉴定用于保护性免疫的免疫原 免疫印迹和色谱技术。 D. 抗Sm-200抗体在吡喹酮治疗中的作用 在 被动转移研究针对200 kD的单克隆抗体， 结核糖蛋白被证明可以恢复u-的能力 抑制小鼠消除蠕虫时， 吡喹酮。