MOLECULAR STUDIES OF THE GENOME AND SURFACE OF SCHISTOSOMA MANSONI

曼索尼血吸虫基因组和表面的分子研究

• 批准号: 3960497
• 负责人: A SHER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3960497
• 关键词: anthelmintics; bacteriophage lambda; biological polymorphism; complementary DNA; gene expression; genetic library; genetic manipulation; genetic markers; genome; helminthic antigen; immunological substance; molecular biology; molecular cloning; monoclonal antibody; nucleic acid sequence; parasitic disease chemotherapy; schistosomiasis; surface antigens

The aim of this project is to characterize schistosome surface molecules relevant to immunity, to analyze the genome of the parasite in relation to specific biologic properties of the organism and to clone important schistosome immunogens. A. Cloning and nucleotide sequencing of the Sm-97 vaccine antigen. Two clones expressing epitopes of the SM-97 vaccine immunogen were obtained from a lambda gt-11 expression library. One of these clones corresponding to 60% of the native molecule was sequenced and the protein deduced to be an alpha-helical coiled coil protein with a structure and amino acid sequence similar to that of paramyosin -- an invertebrate muscle protein. The identity of Sm-97 as paramyosin was confirmed by purifying from schistosomes by conventional chemical procedures and demonstrating identify with Sm-97 by Western blotting. B. Identification of a RFLP associated with drug resistance. A restriction fragment length polymorphism (RFLP) was identified in the DNA of schistosomes with experimentally induced resistance to the anti-parasitic drug hycanthone. This RFLP was absent in the susceptible parent line as well as in other susceptible strains.

这个项目的目的是表征一些表面分子 与免疫力有关，分析寄生虫的基因组， 生物体的特定生物学特性，并克隆重要的 一些免疫原。 A. Sm-97疫苗抗原的克隆和核苷酸序列分析 两 获得表达SM-97疫苗免疫原的表位的克隆 从lambda GT-11表达式库。 其中一个克隆体 到60%的天然分子被测序，并推断蛋白质是 一种α-螺旋卷曲螺旋蛋白，其结构和氨基酸 与副肌球蛋白（一种无脊椎动物的肌肉蛋白）相似的序列。 Sm-97作为副肌球蛋白的身份通过从 通过常规化学方法测定样品，并证明鉴别 Western blotting法检测Sm-97的表达。 B。与耐药性相关的RFLP的鉴定。 的限制 片段长度多态性（RFLP）在DNA中被鉴定， 对抗寄生虫药具有实验诱导抗性的噬菌体 药物羟乙孕酮。 该RFLP在感病亲本系中不存在， 以及其他敏感菌株。