ROLE OF TRANSFORMING GROWTH FACTOR IN HEPATOCARCINOGENESISALPHA

转化生长因子α（TGF-α）以高水平表达 在人肝细胞癌（HCC）中，它可能是一种机制的一部分， 哪种肝炎B病毒（HBV）导致HCC。 研究继续在 分析TGF-α在HCC病因中的作用。 以前的工作 在这个实验室中，显示了高水平的TGF-α表达， 人HCC和肝母细胞瘤中TGF-α基因的上调 用B型肝炎病毒（HBV）转染后的细胞系。 随后，进行研究以检查TGF-β 1的表达。 17例肝良性肿瘤患者的免疫组化检测 局灶性结节性增生（FNH），以及16例HCC患者。 所有 这些病人来自匈牙利。 在13例中检测到过表达 HCC患者（81%）和12例FNH患者（71%）。 患者 在FNH中，TGF-α在增殖的小管细胞中强烈染色。 在选定的HCC患者中，连续切片的染色 揭示了TGF-α和B型肝炎表面抗原的表达， 相邻的非肿瘤性肝的相同肝细胞，如前所述 该实验室在美国和中国的HCC中报告。 在日本的HCC患者中进行了额外的研究， 评估TGF-α在不同级别的肿瘤区域的表达， 来自相同患者的组织学严重程度，大概是在以下情况下， 在疾病的过程中会出现更严重的等级。 初步 结果表明，肿瘤的区域或结节具有更多 分化的组织学（可能是不太先进的部分）具有更大的 TGF-α表达。

Transforming growth factor alpha (TGF-alpha) is expressed at high levels in human hepatocellular carcinoma (HCC); it may be part of a mechanism by which hepatitis B virus (HBV) causes HCC. Research has continued in the analysis of the role of TGF-alpha in the etiology of HCC. Previous work in this laboratory had shown high levels of TGF-alpha expression in most human HCCs, and an up-regulation of the TGF-alpha gene in a hepatoblastoma cell line after transfection with the hepatitis B virus (HBV). Subsequently, studies were conducted to examine the expression of TGF- alpha by immunohistochemistry in benign liver tumors in 17 patients with focal nodular hyperplasia (FNH), as well as 16 patients with HCC. All of these patients were from Hungary. Overexpression was detected in 13 patients with HCC (81%) and in 12 patients with FNH (71%). In patients with FNH, TGF-alpha stained strongly in the proliferating ductular cells. In selected patients with HCC, staining of consecutively cut sections revealed the expression of TGF-alpha and hepatitis B surface antigen in the same hepatocytes of adjoining nontumorous liver, as previously reported by this laboratory in HCCs from the United States and China. Additional studies have been conducted in HCC patients from Japan to evaluate TGF-alpha expression in tumor areas of different grades of histologic severity from the same patients, presumably in situations where a more severe grade evolved during the course of the disease. Preliminary results indicate that regions or nodules of the tumors with more differentiated histology (presumably, less advanced portions) have greater TGF-alpha expression.