ROLE OF TRANSFORMING GROWTH FACTOR IN HEPATOCARCINOGENESISALPHA

转化生长因子在肝癌发生中的作用

• 批准号: 3774912
• 负责人: E TABOR
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3774912
• 关键词: Europe; Japan; gene expression; gene induction /repression; hepatitis B virus group; hepatocellular carcinoma; human tissue; hyperplasia; immunocytochemistry; liver cells; liver neoplasms; molecular oncology; neoplastic cell; stainings; tissue /cell culture; transfection; transforming growth factors; viral carcinogenesis; virus antigen; virus infection mechanism

Transforming growth factor alpha (TGF-alpha) is expressed at high levels in human hepatocellular carcinoma (HCC); it may be part of a mechanism by which hepatitis B virus (HBV) causes HCC. Research has continued in the analysis of the role of TGF-alpha in the etiology of HCC. Previous work in this laboratory had shown high levels of TGF-alpha expression in most human HCCs, and an up-regulation of the TGF-alpha gene in a hepatoblastoma cell line after transfection with the hepatitis B virus (HBV). Subsequently, studies were conducted to examine the expression of TGF- alpha by immunohistochemistry in benign liver tumors in 17 patients with focal nodular hyperplasia (FNH), as well as 16 patients with HCC. All of these patients were from Hungary. Overexpression was detected in 13 patients with HCC (81%) and in 12 patients with FNH (71%). In patients with FNH, TGF-alpha stained strongly in the proliferating ductular cells. In selected patients with HCC, staining of consecutively cut sections revealed the expression of TGF-alpha and hepatitis B surface antigen in the same hepatocytes of adjoining nontumorous liver, as previously reported by this laboratory in HCCs from the United States and China. Additional studies have been conducted in HCC patients from Japan to evaluate TGF-alpha expression in tumor areas of different grades of histologic severity from the same patients, presumably in situations where a more severe grade evolved during the course of the disease. Preliminary results indicate that regions or nodules of the tumors with more differentiated histology (presumably, less advanced portions) have greater TGF-alpha expression.

转化生长因子-α高水平表达 在人类肝细胞癌(HCC)中；它可能是通过 哪种乙肝病毒(乙肝)会导致肝细胞癌。研究仍在继续 转化生长因子-α在肝细胞癌病因中的作用分析以前的工作 在本实验室中已显示在大多数细胞中高水平表达转化生长因子-α 人肝细胞癌与肝母细胞瘤中转化生长因子-α基因上调 将乙肝病毒(乙肝病毒)导入细胞系。 随后，进行了研究以检测转化生长因子-1的表达。 17例良性肝肿瘤的免疫组织化学检测 局灶性结节增生(FNH)，以及16例肝细胞癌患者。所有的 这些患者来自匈牙利。在13例中检测到过表达 肝细胞癌患者(81%)和FNH患者(71%)。在病人中 FNH时，转化生长因子-α在增殖的导管细胞中呈强阳性表达。 在选定的肝细胞癌患者中，连续切片染色 揭示了转化生长因子-α和乙肝表面抗原在肝细胞中的表达 与以前相同的邻近非肿瘤性肝脏的肝细胞 由本实验室报道的来自美国的碳氢化合物和中国。 对来自日本的肝癌患者进行了额外的研究，以 不同分化程度肿瘤组织中转化生长因子-α的表达 相同患者的组织学严重程度，推测在以下情况下 在疾病的过程中，出现了更严重的级别。初步 结果表明，肿瘤的区域或结节具有较多的 分化的组织学(可能是较不发达的部分)具有更大的 转化生长因子-α的表达。