HEPATOCELLULAR CARCINOMA IN CHIMPANZEES

黑猩猩肝细胞癌

• 批准号: 3838499
• 负责人: E TABOR
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3838499
• 关键词: Pan; avidin; biotin; immunocytochemistry; molecular oncology; transforming growth factors; tumor suppressor genes

HCC, an occasional outcome of chronic infection with hepatitis B or C viruses (HBV, HCV) in humans, has been described in only three chimpanzees. HCC and adjacent nontumorous liver from autopsies from two of these were studied using an avidin-biotin-complex immunohistochemical method. Both tumors were well-differentiated trabecular HCCs. Neither chimpanzee had active HBV infection; one had chronic non-A, non-B hepatitis (most likely due to HCV). TGF-alpha stained strongly in >70% of HCC cells of both chimpanzees (most other HCC cells were negative). The HCCs of both chimpanzees stained negatively for p53; this probably indicates the absence of mutant p53 protein. However, one chimpanzee had several microscopic foci of hepatocytes in the nontumorous liver that contained a few nuclei (<1%) that stained positively for p53, possibly the result of additional mutational events outside the tumor. Oval cells, characterized by oval nuclei, clear and scanty cytoplasm, and dimensions that are smaller than mature hepatocytes, were observed in liver tissues from both chimpanzees in the present study and were stained strongly positive for TGF-alpha.

HCC，慢性乙型或丙型肝炎感染的偶然结果 人类中的病毒（HBV、HCV）仅在三种 黑猩猩。 来自两个尸体解剖的 HCC 和邻近的非肿瘤肝脏 其中使用亲和素-生物素复合物免疫组织化学进行研究 方法。 两种肿瘤都是分化良好的小梁性肝癌。 两者都不 黑猩猩患有活跃的乙型肝炎病毒感染；一名患有慢性非 A、非 B 肝炎（很可能是由于丙型肝炎病毒）。 两只黑猩猩 >70% 的 HCC 细胞中 TGF-α 强烈染色（大多数 其他 HCC 细胞呈阴性）。 两只黑猩猩的 HCC 均被染色 p53 呈阴性；这可能表明突变体 p53 的缺失 蛋白质。 然而，一只黑猩猩有几个微观病灶 非肿瘤肝脏中的肝细胞含有少量细胞核 (<1%) p53 染色呈阳性，可能是额外的结果 肿瘤外的突变事件。 卵形细胞，其特征为卵圆形 细胞核，透明且稀少的细胞质，尺寸小于 在两只黑猩猩的肝组织中观察到成熟的肝细胞 在本研究中，TGF-α 染色呈强阳性。