ONCOGENE EXPRESSION IN CELL LINES DERIVED FROM HUMAN HEPATOCELLULAR CARCINOMA

人肝细胞癌细胞系中癌基因的表达

• 批准号: 3853543
• 负责人: E TABOR
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3853543
• 关键词: RNA; electrophoresis; embryo /fetus cell /tissue; gene expression; hepatitis B virus group; human tissue; neoplasm /cancer genetics; northern blottings; nucleic acid hybridization; oncogenes; tissue /cell culture; viral carcinogenesis; virus DNA

The possible role of oncogenes in the development of human hepatocellular carcinoma (HCC) has not been well defined. We have examined several cell lines from human HCC, as well as a control (non-HCC) cell line (WI-38) for levels of expression of oncogenes. The expression of thirteen oncogenes (c-myc, H-ras, K-ras, N-ras, raf, Mb, abl, N-myc, erb-A, erb-B, fos, src, and mos) and two tumor suppressor genes (Rb and p53) were studied by northern blot hybridization in three human HCC/HB cell lines (PLC/PRF/5, Hep3B, and Hep G2) and in a human embryonic lung fibroblast cell line (WI-38) to look for differences that might be associated with the presence (PLC/PRF/5 and Hep3B) or absence (Hep G2) of integrated hepatitis B virus (HBV) DNA. Most of the oncogenes were expressed at a higher level (based on band intensity) in HCC/HB cell lines than in WI-38 cell lines (exceptions: K-ras, fos, and Rb were expressed at the same level in all four cell lines; p53 was not detectable in Hep3B). Certain oncogenes (c-myc, H-ras, raf, src) were expressed at different levels among the three HCC/HB cell lines, unrelated to the presence or absence of integrated HBV-DNA. Additional studies were conducted using additional human HCC cell lines: 2.2.15 (which is derived from Hep G2 transfected with HBV) and the three Japanese lines: HLE, HLF, and HuH7. The data for these lines are still being analyzed.

癌基因在人肝细胞癌发生发展中的可能作用 癌症(HCC)的定义还不是很清楚。我们已经检查了几个细胞 人肝细胞癌细胞系和对照(非肝细胞癌)细胞系(WI-38) 了解癌基因的表达水平。 13个癌基因(c-myc、H-ras、K-ras、N-ras、raf、 MB、ABL、N-myc、erb-A、erb-B、fos、src和mos)和两个肿瘤抑制因子 用Northern印迹杂交技术研究了Rb和P53基因在3个基因中的表达 人肝癌/HB细胞系(PLC/PRF/5、Hep3B和Hep G2)和人 胚胎肺成纤维细胞株(WI-38)寻找差异 可能与存在(PLC/PRF/5和Hep3B)或不存在有关 (Hep G2)整合的乙肝病毒(HBVDNA)。大多数 癌基因的表达水平较高(基于条带强度) 肝细胞癌/HB细胞系比WI-38细胞系(K-ras、FOS和 Rb在所有四种细胞系中的表达水平相同，而P53的表达水平不同 在Hep3B中可检测到)。某些癌基因(c-myc、H-ras、raf、src) 在三种肝癌/HB细胞系中不同水平表达， 与整合的HBVDNA的存在与否无关。 使用更多的人肝细胞癌细胞系进行了其他研究： 2.2.15(来源于感染乙肝病毒的Hep G2)和三个 日语台词：HLE、HLF和HUH7。这些行的数据仍然是 正在被分析。