PCR FOR DETECTION OF HEPATITIS B VIRUS IN SERA FROM PATIENTS WITH HCC

检测血清中B型肝炎病毒（HBV）DNA 来自25名日本肝细胞癌（HCC）患者，使用 聚合酶链反应（PCR）方法。 PCR扩增的两个阶段 (30每个循环）对于HBV DNA的三个不同基因片段，PreC/C， S和X基因，进行使用外部和内部引物对。 的 分别扩增三个靶序列（标准PCR），或 同时进行（“多靶点”PCR）。 16份B型肝炎表面抗原（HBsAg）阴性血清中， 所有三个基因片段均为阴性;其余一份血清为阳性 仅针对PreC/C基因区段。 9份HBsAg阳性血清中有8份 所有三个基因片段均呈阳性;其余一种血清可 不能被确定为积极或消极。 其他血清学研究（例如，HBeAg、抗-HBc）。

The presence of hepatitis B virus (HBV) DNA was investigated in the sera from 25 Japanese patients with hepatocellular carcinoma (HCC) using the polymerase chain reaction (PCR) method. Two stages of PCR amplifications (30 cycles each) for three different gene segments of HBV DNA, the PreC/C, S and X genes, were performed using the outer and inner primer pairs. The three target sequences were amplified separately (standard PCR) or simultaneously ("multi-target" PCR). Fifteen of the 16 hepatitis B surface antigen (HBsAg)-negative sera were negative for all three gene segments; the remaining one serum was positive only for the PreC/C gene segment. By contrast, 8 of 9 HBsAg-positive sera were positive for all three gene segments; the remaining one serum could not be determined to be positive or negative. Additional serologic studies (e.g., HBeAg, anti-HBc) were also conducted.