ROLE OF HEPATITIS C VIRUS IN HEPATOCELLULAR CARCINOMA

有人认为，丙型肝炎病毒（HCV）毒株变异可能 解释不同的肝细胞癌（HCC）患病率。 然而，在这方面， 很少有研究探讨了这种变化发生的程度， HCC患者。 1969-1981年从91例HCC患者中获得的血清 来自赞比亚，35名来自乌干达，27名来自美国。 PCR扩增5 ′-非编码区163-175个核苷酸的片段， 通过dsDNA循环测序进行测序。 8份标本中有HCV-RNA （赞比亚7人;乌干达1人;美国0人）。 与原型株HCV-1相比，至少有四种模式的HCV-1， 核苷酸变异。观察到0.5-2.3%的变异， 这个高度保守的病毒基因片段 其中一些可能是 在非洲流行的菌株差异;三个已报告在>50% 在扎伊尔和南非，HCV感染率为20%，在埃及，HCV感染率为50%。 这些数据表明HCC与独特的HCV毒株无关， 尽管HCV基因组中其他地方的关键突变不能 排除 20世纪70年代从25名日本HCC患者中收集的血清样本 对来自夏威夷的后裔和匹配的对照进行HCV抗体检测 以及HCV-RNA的存在，以确定HCV是否与 这些患者中的HCC与日本近十年的情况一样。 抗 在任何HCC患者中均未检测到HCV;在2例患者中检测到HCV-RNA。 虽然这些结果不是决定性的，但它们表明夏威夷的HCC 在20世纪70年代与慢性HCV感染没有高度相关性。 （这 日本的HCC患者也可能如此，因为 目前日本HCV相关HCC的高患病率发生在 25-30年）。

It has been suggested that hepatitis C virus (HCV) strain variations could explain differing hepatocellular carcinoma (HCC) prevalences. However, few studies have examined the extent to which such variations occur in patients with HCC. Sera obtained between 1969-1981 from 91 HCC patients from Zambia, 35 from Uganda, and 27 from the United States were screened. A PCR-amplified 163-175 nucleotide segment of the 5'-noncoding region was sequenced by dsDNA cycle sequencing. Eight samples contained HCV-RNA (seven from Zambia; one from Uganda; none from the United States). Compared with the prototype strain HCV-1, at least four patterns of nucleotide variation were found. Variations of 0.5-2.3% were observed in this highly conserved virus gene segment. Some of these could be prevalent strain differences in Africa; three have been reported in >50% of HCV infections in Zaire and South Africa and two in >50% in Egypt. These data suggest HCC is not associated with a unique HCV strain, although critical mutations elsewhere in the HCV genome cannot be excluded. Serum samples collected in the 1970s from 25 HCC patients of Japanese descent from Hawaii and matched controls were tested for antibody to HCV and for the presence of HCV-RNA to determine whether HCV was associated with HCC in these patients as it is in Japan in the current decade. Anti- HCV was not detected in any HCC patients; HCV-RNA was detected in two. Although these results are not conclusive, they suggest that HCC in Hawaii was not highly associated with chronic HCV infection in the 1970s. (This may also have been true of HCC patients in Japan, since the evolution of the high prevalence of HCV-associated HCC in Japan today occurred during the past 25-30 years.)