EFFECT OF HEPATITIS B VIRUS ON TRANSFORMING GROWTH FACTOR ALPHA

转化生长因子α（TGF-α）以高水平表达 在人肝细胞癌（HCC）中，它可能是一种机制的一部分， 哪种肝炎B病毒（HBV）导致HCC。 为了确定 HBV对TGF-α、转化生长因子表达的直接影响 在人肝母细胞瘤细胞系中检测α因子（TGF-α）， Hep G2不含B型肝炎病毒（HBV）DNA， 细胞系2.2.15，其通过用以下转染Hep G2细胞形成： 完整的HBV DNA，以研究HBV和TGF-α 在肝癌的发生过程中起辅助作用。 北方斑点 从这些细胞系中提取的RNA与密度计杂交， 分析，揭示了TGF-α基因在转染的细胞中的表达。 细胞中的水平比未转染的细胞高三倍。 使用抗TGF-β和抗生物素蛋白的单克隆抗体对细胞进行染色。 生物素-过氧化物酶免疫组化法显示， 转染细胞系中TGF-α染色的强度。 这些 研究结果表明，HBV DNA的存在似乎会导致显著的 TGF-α基因的上调。 这种对TGF-α基因的影响 可能是HBV导致 部分患者发生肝细胞癌。

Transforming growth factor alpha (TGF-alpha) is expressed at high levels in human hepatocellular carcinoma (HCC); it may be part of a mechanism by which hepatitis B virus (HBV) causes HCC. In order to determine the direct effect of HBV on TGF-alpha, the expression of transforming growth factor alpha (TGF-alpha) was examined in a human hepatoblastoma cell line, Hep G2, which does not contain hepatitis B virus (HBV) DNA, and in the cell line 2.2.15 which was formed by the transfection of Hep G2 cells with the complete HBV DNA, to study the possibility that HBV and TGF-alpha could function as co-factors in hepatocarcinogenesis. Northern blot hybridization of RNA extracted from these cell lines, with densitometric analysis, revealed expression of the TGF-alpha gene in the transfected cells at a level three times higher than in the nontransfected cells. Staining of the cells using a monoclonal antibody to TGF-` and the avidin- biotin-peroxidase immunohistochemical method revealed a much higher intensity of TGF-alpha staining in the transfected cell line. These findings show that the presence of HBV DNA appears to cause a significant up-regulation of the TGF-alpha gene. This effect on the TGF-alpha gene may be a mechanism by which HBV contributes to the etiology of hepatocellular carcinoma in some patients.