EFFECT OF HEPATITIS B VIRUS ON TRANSFORMING GROWTH FACTOR ALPHA

乙型肝炎病毒对转化生长因子α的影响

• 批准号: 3838498
• 负责人: E TABOR
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3838498
• 关键词: gene induction /repression; hepatitis B virus group; molecular oncology; neoplastic cell; tissue /cell culture; transforming growth factors; viral carcinogenesis

Transforming growth factor alpha (TGF-alpha) is expressed at high levels in human hepatocellular carcinoma (HCC); it may be part of a mechanism by which hepatitis B virus (HBV) causes HCC. In order to determine the direct effect of HBV on TGF-alpha, the expression of transforming growth factor alpha (TGF-alpha) was examined in a human hepatoblastoma cell line, Hep G2, which does not contain hepatitis B virus (HBV) DNA, and in the cell line 2.2.15 which was formed by the transfection of Hep G2 cells with the complete HBV DNA, to study the possibility that HBV and TGF-alpha could function as co-factors in hepatocarcinogenesis. Northern blot hybridization of RNA extracted from these cell lines, with densitometric analysis, revealed expression of the TGF-alpha gene in the transfected cells at a level three times higher than in the nontransfected cells. Staining of the cells using a monoclonal antibody to TGF-` and the avidin- biotin-peroxidase immunohistochemical method revealed a much higher intensity of TGF-alpha staining in the transfected cell line. These findings show that the presence of HBV DNA appears to cause a significant up-regulation of the TGF-alpha gene. This effect on the TGF-alpha gene may be a mechanism by which HBV contributes to the etiology of hepatocellular carcinoma in some patients.

转化生长因子 α (TGF-α) 高水平表达 在人类肝细胞癌（HCC）中；它可能是机制的一部分 乙型肝炎病毒 (HBV) 会导致 HCC。 为了确定 HBV对转化生长因子TGF-α表达的直接影响 在人肝母细胞瘤细胞系中检查了因子 α（TGF-α）， Hep G2，不含乙型肝炎病毒 (HBV) DNA，并且在 细胞系2.2.15是通过转染Hep G2细胞形成的 完整的 HBV DNA，以研究 HBV 和 TGF-α 存在的可能性 可能作为肝癌发生的辅助因子。 北方印迹 从这些细胞系中提取的 RNA 进行杂交，并进行光密度测定 分析显示转染细胞中TGF-α基因的表达 细胞的水平比未转染的细胞高三倍。 使用 TGF-` 和抗生物素蛋白的单克隆抗体对细胞进行染色 生物素过氧化物酶免疫组化方法显示出更高的 转染细胞系中TGF-α染色的强度。 这些 研究结果表明，HBV DNA 的存在似乎会导致显着的 TGF-α基因的上调。 对 TGF-α 基因的影响 可能是 HBV 导致乙型肝炎病因的一种机制 部分患者发生肝细胞癌。