ENZYME STRUCTURE

酶结构

• 批准号: 3776343
• 负责人: D R DAVIES
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3776343
• 关键词: Salmonella typhimurium; X ray crystallography; active sites; enzyme complex; enzyme mechanism; enzyme structure; mutant; tryptophan synthase

The multi-enzyme complex, tryptophan synthase from Salmonella typhimurium, has been further analyzed by X-ray diffraction. High resolution data have been collected for two derivatives of a mutant, K87T, of the beta subunit that approximate enzyme catalyzed intermediates. These structures have been refined, to provide new information about the disposition of residues and their interaction with the intermediates of the beta reaction. In addition another mutant, alpha60E, has been analyzed and data have been collected for two inhibitor complexes of the betaK87T mutant to examine the effect on flexible regions of the alpha active site.

沙门氏菌色氨酸合成酶 鼠伤寒沙门氏菌，已通过X射线衍射进一步分析。 高 已经收集了突变体的两种衍生物的分辨率数据， K87 T，是一种近似酶催化的β亚基， 中间体的 这些结构已被完善，以提供新的 关于残留物的处置及其与 β反应的中间产物 另外一个变种， alpha60E，已被分析和数据已收集了两个 β K87 T突变体的抑制剂复合物，以检查对 α活性位点的柔性区域。