ENZYME STRUCTURE

酶结构

• 批准号: 3875894
• 负责人: D R DAVIES
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3875894
• 关键词: X ray crystallography; calcium binding protein; crystallization; enzyme inhibitors; enzyme structure; molecular biology; mutant; peptidases; placenta; site directed mutagenesis; stereochemistry; tryptophan synthase

1) The crystal structure of the bifunctional enzyme complex,tryptophan synthase, has been refined at 2.4A resolution. 2) Two inhibitor complexes of the Rhizopuspepsin have been analyzed by X-ray diffraction. Both complexes have been refined and the structures analyzed in terms of the probable mechanism of action. 3) Endonexin II, a calcium- and phospholipid-binding protein isolated from human placenta, has been successfully crystallized. X-ray diffraction data have been collected from native and heavy-atom treated crystals, and their space group has been determined.

1)双功能酶复合物色氨酸的晶体结构 合成酶，已在2.4A分辨率下精制。 2)已分析了根霉胃蛋白酶的两种抑制剂复合物， x射线衍射这两种配合物都经过了改进， 根据可能的作用机制进行分析。 3)Endonexin II是一种从大肠杆菌中分离的钙和磷脂结合蛋白， 人类胎盘已经被成功地结晶化了x射线衍射数据 已经从天然和重原子处理的晶体中收集， 空间群已确定。