THREE-DIMENSIONAL STRUCTURE OF PROTEINS OF THE IMMUNE SYSTEM

免疫系统蛋白质的三维结构

• 批准号: 3940693
• 负责人: D R DAVIES
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3940693
• 关键词: X ray crystallography; computer simulation; conformation; crystallization; galactans; haptens; immunoglobulin E; immunoglobulin structure; isomorphous substitution; lysozyme; molecular biology; monoclonal antibody; myeloma globulin; phosphorylcholine

1) The high resolution structure of J539 has been refined. All the amino acid residues have been located with the exception of three that appear to be disordered. 2) The three-dimensional structure of two crystal forms of a complex between lysozyme and the Fab of a monoclonal antibody to lysozyme have been determined. The method used was molecular replacement in which the known structures of the Fab and of lysozyme were used as probes to determine their orientations and positions in the new crystal.

1)J539的高分辨率结构已经得到改进。 所有的 氨基酸残基已被定位，除了三个 看起来很混乱 2)两种晶型的三维结构 溶菌酶与单克隆抗体Fab之间的复合物 对溶菌酶的影响。 使用的方法是 分子置换，其中Fab的已知结构 和溶菌酶作为探针， 新晶体中的方向和位置。